Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2015; 21(5): 1479-1487
Published online Feb 7, 2015. doi: 10.3748/wjg.v21.i5.1479
Xiaotan Sanjie decoction inhibits interleukin-8-induced metastatic potency in gastric cancer
Jun Shi, Pin-Kang Wei
Jun Shi, Pin-Kang Wei, Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China
Author contributions: Wei PK designed the research; Shi J performed the research and wrote the paper.
Supported by Grant from the three-year action plan fund of Traditional Chinese Medicine, Shanghai City Health Administration, China, No. ZYSNXD-CC-ZDYJ024.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Pin-Kang Wei, Professor, Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, No.415 Fengyang Road, Huangpu District, Shanghai 200003, China. czzyk73408@163.com
Telephone: +86-21-81885471 Fax: +86-21-63520020
Received: August 4, 2014
Peer-review started: August 5, 2014
First decision: August 27, 2014
Revised: September 9, 2014
Accepted: October 15, 2014
Article in press: October 15, 2014
Published online: February 7, 2015
Abstract

AIM: To investigate the interaction between Xiaotan Sanjie (XTSJ) decoction and interleukin-8 (IL-8) and its effect on adhesion, migration and invasion of SGC-7901 gastric cancer cells.

METHODS: SGC-7901 gastric cancer cells were exposed to serum containing XTSJ decoction and/or IL-8 (1 ng/mL). SGC-7901 cell adhesion to fibronectin, an extracellular matrix component, was detected using the Cell Counting Kit-8. Migration and invasion abilities of SGC-7901 cells were detected by scratch wound and Transwell chamber assays. Then, protein (immunofluorescence and Western blot) and mRNA levels (quantitative polymerase chain reaction) of cluster of differentiation 44 (CD44), a cell adhesion molecule, were measured in 72-h-cultured SGC-7901 cells.

RESULTS: Cell adhesion was promoted by IL-8 (P = 0.001), but was inhibited by XTSJ decoction (P = 0.0001). Similarly, IL-8 promoted SGC-7901 cell invasion (P = 0.003), and XTSJ decoction inhibited cell invasion (P = 0.001). IL-8 induced SGC-7901 cell migration, but this was inhibited by XTSJ decoction. IL-8 up-regulated CD44 protein (P = 0.028) and mRNA expression (P = 0.002), whereas XTSJ decoction inhibited CD44 protein expression (P = 0.0001), but not mRNA expression (P = 0.275). An interaction between XTSJ decoction and IL-8 was confirmed in the invasion (P = 0.001) and CD44 mRNA expression of SGC-7901 cells (P = 0.010), but not in cell adhesion (P = 0.051).

CONCLUSION: XTSJ decoction may inhibit adhesion, migration and invasion of gastric cancer cells, which is partly associated with down-regulation of IL-8.

Keywords: Xiaotan Sanjie decoction, Interleukin-8, Migration, Adhesion, Invasion, Cluster of differentiation 44, Gastric cancer SGC-7901 cell line

Core tip: SGC-7901 gastric cancer cells were used to evaluate the effects of Xiaotan Sanjie (XTSJ) decoction on adhesion, migration and invasion of gastric cancer cells and the role of interleukin-8 (IL-8) in these effects in vitro. This study focused on the interaction between XTSJ decoction and IL-8. XTSJ decoction significantly inhibited adhesion, migration and invasion of SGC-7901 cells, which was partly associated with down-regulation of IL-8. The results reflect the therapeutic potential of XTSJ decoction for preventing metastasis of gastric cancer.