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©The Author(s) 2015.
World J Gastroenterol. Aug 28, 2015; 21(32): 9544-9553
Published online Aug 28, 2015. doi: 10.3748/wjg.v21.i32.9544
Published online Aug 28, 2015. doi: 10.3748/wjg.v21.i32.9544
Table 1 Changes of portal vein in experimental group 2 before and after perfusion
| Portal venous diameter (mm) | Pressure (kPa) | Extrahepatic portal venous bypass | Intrahepatic portal venous bypass |
| Before perfusion (n = 5) | |||
| 1 | 10.3 | 1.4 | -- |
| 1 | 9.8 | 1.5 | -- |
| 1 | 11.4 | 1.4 | -- |
| 1 | 12.2 | 1.6 | -- |
| 1 | 10.0 | 1.5 | -- |
| mean ± SD | 10.74 ± 1.02 | 1.48 ± 0.84 | |
| Immediately after perfusion (n = 5) | |||
| 1 | 18.2 | 2.5 | -- |
| 1 | 17.1 | 2.7 | -- |
| 1 | 20.2 | 2.6 | -- |
| 1 | 16.7 | 2.9 | -- |
| 1 | 18.4 | 2.4 | -- |
| mean ± SD | 18.12 ± 1.37 | 2.62 ± 0.19 | |
| 2 wk after perfusion (n = 5) | |||
| 1 | 13.6 | 1.8 | -- |
| 1 | 12.7 | 2.1 | -+ |
| 1 | 14.5 | 2.0 | -+ |
| 1 | 13.3 | 2.4 | -- |
| 1 | 12.9 | 1.9 | -+ |
| mean ± SD | 13.40 ± 0.71 | 2.04 ± 0.23 | |
| 4 wk after perfusion (n = 5) | |||
| 1 | 12.3 | 1.6 | -+ |
| 1 | 11.8 | 1.9 | -+ |
| 1 | 14.1 | 1.5 | -+ |
| 1 | 13.1 | 2.1 | -+ |
| 1 | 11.7 | 1.7 | -+ |
| mean ± SD | 12.60 ± 1.00 | 2.04 ± 0.23 | |
| 6 wk after perfusion (n = 5) | |||
| 1 | 11.5 | 1.7 | -+ |
| 1 | 11.9 | 1.8 | -+ |
| 1 | 16.1 | 1.4 | -+ |
| 1 | 16.5 | 2.1 | -+ |
| 1 | 11.8 | 1.9 | -+ |
| mean ± SD | 13.56 ± 2.51 | 1.78 ± 0.26 |
Table 2 Changes of portal vein in experimental group 3 before and after perfusion
| Portal venous diameter (mm) | Pressure (kPa) | Extrahepatic portal venous bypass | Intrahepatic portal venous bypass |
| Before perfusion (n = 5) | |||
| 1 | 9.5 | 1.2 | -- |
| 1 | 10.3 | 1.6 | -- |
| 1 | 11.5 | 1.4 | -- |
| 1 | 9.8 | 1.3 | -- |
| 1 | 12.4 | 1.5 | -- |
| mean ± SD | 10.70 ± 1.22 | 1.40 ± 0.16 | |
| Immediately after perfusion (n = 5) | |||
| 1 | 19.4 | 3.1 | -- |
| 1 | 18.6 | 3.3 | -- |
| 1 | 21.1 | 3.5 | -- |
| 1 | 17.9 | 2.8 | -- |
| 1 | 19.7 | 2.9 | -- |
| mean ± SD | 19.34 ± 1.21 | 3.12 ± 0.29 | |
| 2 wk after perfusion (n = 4) | |||
| 1 | 14.2 | 2.4 | -+ |
| 1 | 15.4 | 2.6 | -+ |
| 1 | 16.3 | 2.8 | -- |
| 1 | 16.6 | 2.6 | -+ |
| mean ± SD | 15.80 ± 3.66 | 2.60 ± 0.16 | |
| 4 wk after perfusion (n = 3) | |||
| 1 | 11.4 | 2.2 | -+ |
| 1 | 16.5 | 1.8 | -+ |
| 1 | 17.9 | 2.1 | -+ |
| mean ± SD | 15.63 ± 1.08 | 2.03 ± 0.21 | |
| 6 wk after perfusion (n = 3) | |||
| 1 | 11.6 | 2.1 | -+ |
| 1 | 18.3 | 1.7 | -+ |
| 1 | 17.5 | 1.9 | -+ |
| mean ± SD | 15.27 ± 3.36 | 1.90 ± 0.20 |
Table 3 Changes of portal vein in experimental group 1 before and after perfusion
| Portal venous diameter (mm) | Pressure (kPa) | Extrahepatic portal venous bypass | Intrahepatic portal venous bypass |
| Before perfusion (n = 5) | |||
| 1 | 10.5 | 1.5 | -- |
| 1 | 10.3 | 1.7 | -- |
| 1 | 10.6 | 1.7 | -- |
| 1 | 11.4 | 1.6 | -- |
| 1 | 10.2 | 1.6 | -- |
| mean ± SD | 10.60 ± 0.47 | 1.62 ± 0.84 | |
| Immediately after perfusion (n = 5) | |||
| 1 | 10.5 | 1.6 | -- |
| 1 | 10.8 | 1.9 | -- |
| 1 | 10.9 | 1.6 | -- |
| 1 | 11.4 | 1.5 | -- |
| 1 | 10.5 | 1.8 | -- |
| mean ± SD | 10.82 ± 0.37 | 1.68 ± 0.16 | |
| 2 wk after perfusion (n = 5) | |||
| 1 | 11.0 | 1.7 | -- |
| 1 | 10.6 | 1.8 | -- |
| 1 | 11.1 | 1.7 | -- |
| 1 | 10.9 | 1.7 | -- |
| 1 | 11.2 | 1.6 | -- |
| mean ± SD | 10.98 ± 0.23 | 1.70 ± 0.07 | |
| 4 wk after perfusion (n = 5) | |||
| 1 | 11.3 | 1.6 | -- |
| 1 | 10.4 | 1.9 | -- |
| 1 | 10.7 | 1.5 | -- |
| 1 | 11.6 | 1.5 | -- |
| 1 | 11.5 | 1.8 | -- |
| mean ± SD | 11.10 ± 0.52 | 1.66 ± 0.18 | |
| 6 wk after perfusion (n = 5) | |||
| 1 | 11.7 | 1.6 | -- |
| 1 | 11.1 | 1.8 | -- |
| 1 | 10.3 | 1.4 | -- |
| 1 | 11.0 | 1.6 | -- |
| 1 | 11.4 | 1.8 | -- |
| mean ± SD | 15.27 ± 3.36 | 1.64 ± 0.17 |
Table 4 Changes of portal vein in control groups before and after perfusion
| Portal venous diameter (mm) | Pressure (kPa) | Extrahepatic portal venous bypass | Intrahepatic portal venous bypass |
| Before perfusion (n = 6) | |||
| 1 | 10.3 | 1.3 | -- |
| 1 | 10.1 | 1.7 | -- |
| 1 | 10.7 | 1.5 | -- |
| 1 | 10.2 | 1.8 | -- |
| 1 | 9.6 | 1.5 | -- |
| 1 | 9.9 | 1.6 | -- |
| mean ± SD | 10.13 ± 0.37 | 1.57 ± 0.18 | |
| Immediately after perfusion (n = 6) | |||
| 1 | 10.3 | 1.4 | -- |
| 1 | 10．2 | 1.6 | -- |
| 1 | 10.7 | 1.5 | -- |
| 1 | 10.3 | 1.8 | -- |
| 1 | 9.7 | 1.6 | -- |
| 1 | 10.2 | 1.5 | -- |
| mean ± SD | 10.24 ± 0.32 | 1.57 ± 0.14 | |
| 2 wk after perfusion (n = 5) | |||
| 1 | 10.1 | 1.7 | -- |
| 1 | 10.3 | 1.8 | -- |
| 1 | 10.4 | 1.7 | -- |
| 1 | 10.6 | 1．7 | -- |
| 1 | 9.9 | 1.6 | -- |
| 1 | 10.3 | 1.7 | -- |
| mean ± SD | 10.27 ± 0.24 | 1.62 ± 0.12 | |
| 4 wk after perfusion (n = 6) | |||
| 1 | 10.4 | 1.4 | -- |
| 1 | 10.5 | 1.7 | -- |
| 1 | 10.4 | 1.6 | -- |
| 1 | 10.8 | 1.7 | -- |
| 1 | 9.8 | 1．5 | -- |
| 1 | 10.6 | 1.6 | -- |
| mean ± SD | 10.42 ± 0.34 | 1.58 ± 0.12 | |
| 6 wk after perfusion (n = 6) | |||
| 1 | 10.6 | 1.6 | -- |
| 1 | 10.3 | 1.8 | -- |
| 1 | 10.2 | 1.5 | -- |
| 1 | 10.6 | 1.6 | -- |
| 1 | 10.1 | 1.7 | -- |
| 1 | 10.4 | 1.7 | -- |
| mean ± SD | 10.37 ± 0.21 | 1.65 ± 0.10 |
Table 5 Changes of liver size and spleen size in experimental group 1 (5 pigs), experimental group 2 (5 pigs), experimental group 3 (3 pigs) and control groups (6 pigs) before perfusion and at 6 wk after perfusion
| Liver size (cm3) | Spleen (cm3) | Avoirdupois (kg) | |
| Experimental groups 2 + 3 | |||
| Before perfusion | 508 ± 67 | 113 ± 38 | 32.9 ± 3.8 |
| 6 wk after perfusion | 589 ± 73 | 159 ± 46 | 40.7 ± 3.5 |
| Experimental group 1 + control groups | |||
| Before perfusion | 479 ± 85 | 102 ± 47 | 33.8 ± 2.9 |
| After perfusion | 504 ± 73 | 111 ± 39 | 39.5 ± 3.5 |
Table 6 Histopathological changes of hepatic biopsy samples within 6 h after perfusion with different concentrations of alcohol
| Group | Venous hepatocyte thrombosis | Hepatocyte degeneration | Inflammatory cell necrosis | Endothelial cell infiltration injury |
| Experimental group 1 (n = 5) | 3 | 1 | 0 | 1 |
| Experimental group 2 (n = 5) | 5 | 4 | 2 | 3 |
| Experimental group 3 (n = 5) | 5 | 5 | 4 | 5 |
| Control group (n = 6) | 0 | 0 | 0 | 0 |
- Citation: Wang L, He FL, Liu FQ, Yue ZD, Zhao HW. Establishment of a hepatic cirrhosis and portal hypertension model by hepatic arterial perfusion with 80% alcohol. World J Gastroenterol 2015; 21(32): 9544-9553
- URL: https://www.wjgnet.com/1007-9327/full/v21/i32/9544.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i32.9544