NOB1 is essential for the survival of RKO colorectal cancer cells

Table 1 Primers used in this study

Genes	Sequence	Length (bp)
GAPDH-F	TGACTTCAACAGCGACACCCA	121
GAPDH-R	CACCCTGTTGCTGTAGCCAAA
NOB1-F	ATCTGCCCTACAAGCCTAAAC	184
NOB1-R	TCCTCCTCCTCCTCCTCAC
BAX-F	TGCTTCAGGGTTTCATCCA	296
BAX-R	GGCCTTGAGCACCAGTTT
WNT7B-F	TCCACTGGTGCTGCTTCG	300
WNT7B-R	GTCACGGGTGCTGTTCTGC

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 Gene Ontology enrichment analysis showing NOB1-targeted pathways and genes involved in cancer development

Gene ontology	Genes	P value	Fold enrichment	FDR
WNT pathway	WNT4, WNT7B, WNT5B, MITF, LEF1, WNT9A, TCF7L1, WNT8B, APC	4.32 × 10-8	16.9523	7.00 × 10-5
Proliferation	PRKCA, CEBPA, FGFR3, IL-8, FGF9, MITF, TGFBR2, EGLN3, NFKBIA, CDKN1A, CASP3, BAX, VEGFA, FGF1, EGF, APC	2.03 × 10-7	5.0931	3.28 × 10-4
Apoptosis	PRKCA, TRAF1, MITF, MLH1, NFKBIA, FADD, BIRC3, DAPK3, CDKN1A, CASP3, NTRK1, BAX, VEGFA, FAS, TRAF4, APC	2.67 × 10-7	4.9854	4.33 × 10-4
FGF pathway	FGF5, FGFR3, FGF9, FGF16, FGF1	4.64 × 10-6	43.1928	0.007516
Angiogenesis	IL-8, EPAS1, FGF9, VEGFA, TGFBR2, EGF, FGF1	2.33 × 10-5	11.8488	0.037727

FDR: False discovery rate; APC: Adenomatous polyposis coli; IL: Interleukin; VEGFA: Vascular endothelial growth factor A; FGF: Fibroblast growth factor; TGF: Transforming growth factor.