Beneficial effects of adenosine triphosphate-sensitive K+ channel opener on liver ischemia/reperfusion injury

Table 1 Effect of Diazoxide treatment on hepatic ischemia/reperfusion

	Control	Saline	Diazoxide
Liver MDA (nmol/mg prot)	1.49 ± 0.20a	3.24 ± 0.26	2.60 ± 0.19
Lung EBD (µg/g dry weight)	70.13 ± 10.19a	200.02 ± 54.44	155.70 ± 43.44
Lung MPO Activity/g tissue	0.032 ± 0.007a	0.062 ± 0.008	0.065 ± 0.012
Serum creatinine1	0.3 ± 0.1a	0.8 ± 0.1	0.9 ± 0.1
Serum Creatinine2	0.3 ± 0.1a	1.0 ± 0.1	0.8 ± 0.2

In Control group, rats underwent laparotomy and liver reperfusion. In Saline and Diazoxide groups, rats were submitted to liver ischemia/reperfusion and treated with saline or diazoxide 15 minutes before liver ischemia. Malonyldialdehyde (MDA) was analyzed in liver tissue and expressed as nmol/mg protein. Lung microvascular permeability was evaluated through Evans blue dye (EBD) extravasation and lung myeloperoxidase (MPO) activity was used evaluation of lung neutrophil infiltration. Creatinine levels in serum are expressed as U/L (1: 4 h after reperfusion; 2: 24 h after reperfusion). Data are expressed as mean ± SEM of 10 animals per group.

^aP < 0.05 vs Control group.