Distribution of the P2X2 receptor and chemical coding in ileal enteric neurons of obese male mice (ob/ob)

Table 1 Characteristics of primary and secondary antibodies

Tissue antigen	Host	Dilution	Code and reference
P2X2 receptor	Rabbit	1:120	AB5244, Chemicon
Nitric oxide synthase	Sheep	1:2000	H205[38]
Choline acetyltransferase	Goat	1:50	AB144P, Chemicon
Calretinin	Goat	1:100	CG1, Swant
Donkey anti-rabbit IgG Alexa 488		1:500	Molecular probes
Donkey anti-sheep IgG Alexa 594		1:100	Molecular probes

CG: Control group.

Table 2 Body weight (g), small intestine length (cm²) and glucose level in control and obese male mice

	Control group	Obese group
Body weight (g)	32.5 ± 0.7	64.7 ± 1a
Small intestine area (cm2)	28.4 ± 5.2	40 ± 3.8a
Glucose (mg/dL)	224.7 ± 50.1	195.5 ± 93.5

Statically different (^aP < 0.05 vs control group) by Student’s t-tests.