Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice

Table 1 Assessment of clinical symptoms and morphological alterations after dextran sulphate sodium administration in Balb/c mice

Group	n	Length of colon (cm)	Colon weight (mg)	Weight of colon/ length of colon	Spleen weight (mg)	Body weight (g)	Assessment of faeces	Rectal bleeding	Mortality
Control	12	7.8 ± 0.78	337.6 ± 69.04	44.26 ± 7.11	97.1 ± 31.35	21.9 ± 3.89	2.5 ± 0.52	0/12	0/12
								(0%/100%)	(0%/100%)
Acute colitis	8	5.9 ± 0.97a	321.9 ± 55.29	55.03 ± 6.48a	197.9 ± 93.58a	17.0 ± 2.30a	5.0 ± 0.85a	5/8	0/8
								(62%/100%)	(0%/100%)
Chronic colitis	8	7.2 ± 0.27e	354.2 ± 69.29	49.39 ± 6.28	119.0 ± 20.74e	18.8 ± 1.06ce	4.2 ± 0.45ce	0/8	0/8
								(0%/100%)	(0%/100%)

Colon inflammation was induced by the administration of 3.5% DSS solution in Balb/c mice. Assessment of faeces was performed using the Bristol scale (points 1 to 7). Rectal bleeding - number of mice with rectal bleeding (percentages)/total number of mice (percentages). Mortality - number of dead mice (percentages)/total number of mice (percentages).

^aP < 0.05, acute DSS-induced colitis groups vs control;

^cP < 0.05, chronic DSS-induced colitis groups vs control;

^eP < 0.05, acute DSS-induced colitis vs chronic DSS-induced colitis groups. Results are presented as mean ± SE.