Ramonaite R, Skieceviciene J, Juzenas S, Salteniene V, Kupcinskas J, Matusevicius P, Borutaite V, Kupcinskas L. Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice. World J Gastroenterol 2014; 20(35): 12533-12541 [PMID: 25253955 DOI: 10.3748/wjg.v20.i35.12533]
Corresponding Author of This Article
Limas Kupcinskas, MD, Professor, Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, A. Mickeviciaus str. 9, Kaunas LT 44307, Lithuania. kupcinskas@gmail.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Original Article
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World J Gastroenterol. Sep 21, 2014; 20(35): 12533-12541 Published online Sep 21, 2014. doi: 10.3748/wjg.v20.i35.12533
Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice
Rima Ramonaite, Jurgita Skieceviciene, Simonas Juzenas, Violeta Salteniene, Juozas Kupcinskas, Paulius Matusevicius, Vilmante Borutaite, Limas Kupcinskas
Rima Ramonaite, Jurgita Skieceviciene, Simonas Juzenas, Violeta Salteniene, Juozas Kupcinskas, Limas Kupcinskas, Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT 44307, Lithuania
Juozas Kupcinskas, Limas Kupcinskas, Department of Gastroenterology, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT 50161, Lithuania
Paulius Matusevicius, Department of Animal Nutrition, Academy of Veterinary, Lithuanian University of Health Sciences, Kaunas LT 47181, Lithuania
Vilmante Borutaite, Institute for Neurosciences, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas LT 44307, Lithuania
Author contributions: Ramonaite R and Skieceviciene J performed the experiments, analysed the data, interpreted the results, prepared the figures, and drafted the manuscript; Juzenas S and Salteniene V performed the experiments and analysed the data; Matusevicius P and Kupcinskas J interpreted the results, analysed the data, and drafted the manuscript; Borutaite V and Kupcinskas L contributed to the conception and design of the research, interpreted the results, and approved the final version of the manuscript.
Correspondence to: Limas Kupcinskas, MD, Professor, Institute for Digestive Research, Academy of Medicine, Lithuanian University of Health Sciences, A. Mickeviciaus str. 9, Kaunas LT 44307, Lithuania. kupcinskas@gmail.com
Telephone: +37-37-326508 Fax: + 37-37-326508
Received: December 17, 2013 Revised: February 11, 2014 Accepted: June 12, 2014 Published online: September 21, 2014
Core Tip
Core tip: Reactive oxygen species (ROS)-induced oxidative stress is one of the most important etiological factors involved in the inflammation. The key producers of ROS in cells are nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzymes. Several studies have shown that epithelial NADPH oxidase might be responsible for normal immune response to antigens in the gut. On the other hand, little is known about the molecular pathways controlling ROS production via NADPH oxidase in primary intestinal epithelial cells during inflammation. The aim of this study was to investigate the role of NADPH oxidase in colon epithelial cells in the pathogenesis of acute and chronic colon inflammation using a mouse model of dextran sulphate sodium-induced colitis. The results of our study revealed the importance of NADPH oxidase in the pathogenesis of colon inflammation.
