Original Article
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Aug 7, 2014; 20(29): 10071-10081
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
Table 1 Relationships between KISS1 methylation and expression and clinicopathological characteristics in colorectal cancer patients
Clinicopathological traitsnMethylated (n)RateP value1RQ3P value2Gray value4P value2
Age (yr) ≤ 60786583.33%0.6020.445 ± 0.0620.3670.534 ± 0.0470.136
> 60484083.33%0.359 ± 0.0750.420 ± 0.059
GenderMale655381.54%0.5770.345 ± 0.0860.2300.423 ± 0.0470.071
Female615285.25%0.489 ± 0.0830.560 ± 0.058
LocationProximal675785.07%0.5760.453 ± 0.0730.2610.466 ± 0.0510.544
Distal594881.36%0.388 ± 0.0920.512 ± 0.055
Size (cm)< 3585086.21%0.4240.442 ± 0.0850.3630.536 ± 0.0490.130
> 3685580.88%0.343 ± 0.0830.421 ± 0.056
Histology well463167.39%0.001a0.546 ± 0.0950.016a0.575 ± 0.0780.134
Moderate, poor807492.50%0.243 ± 0.0850.451 ± 0.041
Depth of invasionT1 + T2442761.35%0.001a0.963 ± 0.1120.001a0.875 ± 0.0470.001a
T3 + T4827895.12%0.223 ± 0.0540.341 ± 0.029
Lymph node metastasisYes484593.75%0.014a0.168 ± 0.0580.001a0.292 ± 0.0340.001a
No786076.92%0.682 ± 0.1050.631 ± 0.050
Distant metastasisYes1111100.00%0.019a0.024 ± 0.0120.001a0.151 ± 0.0310.014a
No1159481.74%0.486 ± 0.0890.512 ± 0.038
Table 2 Methylation-specific polymerase chain reaction primers and real-time polymerase chain reaction primers for KISS1
Primer nameSequenceAnnealing temp (°C)Product (bp)
KISS1 MSP primer5’-AAAGTTTCGTTTCGGAGGGTTC-3’58172
5’-CTTTTATAAAACCCGAAATAACG-3’
KISS1 un-MSP primers5’-AAAGTTTTGTTTTGGAGGGTTT-3’58173
5’-AAAGTTTTGTTTTGGAGGGTTT-3’
KISS1 real-time PCR primers5’-ACCTGGCTCTTCTCACCAAG-3’60201
5’-TAGCAGCTGGCTTCCTCTC-3’
β-actin primers5’-ATCTGGCACCACACCTTCTACAATGAGCTGCG-3’60837
5’-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3’
Table 3 Invasion and migration of various human colorectal cancer cell lines
Human CRC cellsHCT116SW1116SW480VoLoFP value
Invasion38.93 ± 2.8920.29 ± 1.79a11.14 ± 2.19a6.77 ± 0.79a143.4860.001
Migration48.12 ± 2.5437.21 ± 2.92a34.35 ± 2.08a28.41 ± 1.85a36.0580.001