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World J Gastroenterol. Aug 7, 2014; 20(29): 10071-10081
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
KISS1 methylation and expression as predictors of disease progression in colorectal cancer patients
Shao-Qin Chen, Zhi-Hua Chen, Su-Yong Lin, Qi-Bao Dai, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China
Leng-Xi Fu, Rui-Qing Chen, Central Laboratory of Cancer Institute, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China
Author contributions: All authors contributed equally to the work.
Supported by Natural Science Fund of Fujian Province, No. 2013-J01291; and National Key Construction Projects Clinical Specialties
Correspondence to: Shao-Qin Chen, MD, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Fujian Medical University, Chating Street, 20, Taijian, Fuzhou 350005, Fujian Province, China. chenshaoqin@medmail.com.cn
Telephone: +86-591-87982080 Fax: +86-591-87982082
Received: November 26, 2013
Revised: February 24, 2014
Accepted: March 4, 2014
Published online: August 7, 2014
Revised: February 24, 2014
Accepted: March 4, 2014
Published online: August 7, 2014
Core Tip
Core tip:KISS1 promoter methylation and expression were measured in colorectal cancer (CRC) samples to determine the effect of aberrant methylation of the KISS1 promoter during the development of CRC. We determined that KISS1 hypermethylation occurred frequently in CRC and was associated with low KISS1 expression. KISS1 methylation was associated with tumor differentiation, the depth of invasion, lymph node metastasis and distant metastasis. We treated the human HCT116 colorectal carcinoma cell line, which exhibits KISS1 promoter hypermethylation and poor KISS1 expression, with 5-aza-2’-deoxycytidine in vitro. After treatment, we observed re-expression of the KISS1 gene and decreased cell migration.