Brief Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 14, 2013; 19(46): 8659-8670
Published online Dec 14, 2013. doi: 10.3748/wjg.v19.i46.8659
Table 1 Oligonucleotide sequences
RT-PCR of IL10RA and IL10RB of full length coding regions
IL10RA-5’UTR-FP:GTCCCAGCCCAAGGGTAG[NM_001558.3; start: + 5]
IL10RA-3’UTR-RP:CACCCACATACCCTGCACTA[NM_001558.3; start: + 2027]
IL10RB-5’UTR-FP:GTCGTGTGCTTGGAGGAAG[NM_000628.3; start: + 57]
IL10RB-3’UTR-RP:GTGGCTAAGTCCAGGGTCTG[NM_000628.3; start: + 1223]
Sequence analysis of IL10RA and IL10RB messenger/real time RT-PCR quantification analysis
IL10RA-5’UTRb-FP:TCAGACGCTCATGGGACA[NM_001558.3; start: + 132]
IL10RA-3’UTRb-RP:CCCAGTGGACTTGCAGAAA[NM_001558.3; start: + 1938]
IL10RA-3cFP:AACTGGACCGTCACCAACAC[NM_001558.3; start: + 405]
IL10RA-4cRP:AATCTTCCCGAGGATGAAGC[NM_001558.3; start: + 506]
IL10RA-6cFP:AGCTACCCAGTGTCCTGCTC[NM_001558.3; start: + 871]
IL10RA-7cRP:CAAAAAGGCCTCCTCATCAA[NM_001558.3; start: + 983]
IL10RB-5’UTRb-FP:CATGGCGTGGAGCCTT[NM_000628.3; start: + 99]
IL10RB-3’UTRb-RP:GATGGTCTTGGCCCTTGTT[NM_000628.3; start: + 1177]
IL10RB-4cFP:GTGCAATACTGGAAAAACGGT[NM_000628.3; start: + 565]
IL10RB-5cRP:CCCTCGAACTTGAACACAATAA[NM_000628.3; start: + 678]
Genomic PCR and sequencing
IL10RAp1-FP:GCGGTTTGAGGCTCAGC[NC_ 000011.9; start: + 117856447]
IL10RAp1-RP:CAAGACGGAGGCTGAGGA[NC_ 000011.9; start: + 117857234]
IL10RAp2-FP:CTAGCAGGGGAAGAGCAGC[NC_ 000011.9; start: + 117855574]
IL10RAp2-RP:AACCTTCGTCTCCCAGGTTC[NC_ 000011.9; start: + 117856355]
IL10RAp3-FP:TGAGCCAAGTGACACAGAGG[NC_ 000011.9; start: + 117855023]
IL10RAp3-RP:TTGAACATATACCCTGCTGAAGAG[NC_ 000011.9; start: + 117855810]
IL10RA-1FP:CTGTCAGTCCCAGCCCAA[NC_ 000011.9; start: + 17857104]
IL10RA-1RP:TCTCCACTGGATGGAGAACTTTA[NC_ 000011.9; start: + 117857327]
IL10RA-2FP:TTGGTAAAATTGGGGTCATCA[NC_ 000011.9; start: + 117859029]
IL10RA-2RP:GCCCTCAGGCACTCACTTC[NC_ 000011.9; start: + 117859328]
IL10RA-3FP:AAGCTCGTTTCCAGTGCCTA[NC_ 000011.9; start: + 117860120]
IL10RA-3RP:GGCAGACATGGTGAGCTATG[NC_ 000011.9; start: + 117860439]
IL10RA-4FP:ACAAACCTGTGGCCAAGTTT[NC_ 000011.9; start: + 117863822]
IL10RA-4RP:CACACAAGGGTGCTTCCAG[NC_ 000011.9; start: + 117864202]
IL10RA-5FP:ATCACCTCTAAAGGCCCACC[NC_ 000011.9; start: + 117864629]
IL10RA-5RP:GGATGCAGAGCTATGTGAAGC[NC_ 000011.9; start: + 117864993]
IL10RA-6FP:TTTCATGGGACCAGAGTCCT[NC_ 000011.9; start: + 117866223]
IL10RA-6RP:CTGGCTGGGAGGAAAAGAG[NC_ 000011.9; start: + 117864993]
IL10RA-7.1FP:GCTCTCCTCCTGGGCCT[NC_ 000011.9; start: + 117869338]
IL10RA-7.1RP:CGGCCCTCAGAGTTTTGA[NC_ 000011.9; start: + 117869854]
IL10RA-7.2FP:ACCTGGGAGCAACAGGTG[NC_ 000011.9; start: + 117869775]
IL10RA-7.2RP:CGTGCCTAACTTCTGCCC[NC_ 000011.9; start: + 117870445]
ARMS PCR of the -413G->T IL10-RA promoter mutation
IL10RA-ARMS-FP-N:CCGGCACGCCAGGCAAAAGCGGCTCGGTCG[NC_ 000011.9; start: + 117856738]
IL10RA-ARMS-FP-M:CCGGCACGCCAGGCAAAAGCGGCTCGGTCT[NC_ 000011.9; start: + 117856738]
IL10RA-ARMS-RP:GCCTCCAGTGCCTTCGGATCAA[NC_ 000011.9; start: + 117856897]
Gene copy number quantification of IL10RA gene
IL10RA-4cFP:TCCTCGGGAAGATTCAGCTA[NM_001558.3; start: + 493]
IL10RA-4c2RP:TGCGAATGGCAATCTCATAC[NM_001558.3; start: + 594]
IL10RA-7cFP:ACTGAAGAGCCCCAGTTCCT[NM_001558.3; start: + 1065]
IL10RA-7c2RP:GCTGTCTGTGCTATTGCTGC[NM_001558.3; start: + 1187]
RT-PCR: Reverse transcription polymerase chain reaction; IL10: Interleukin-10.