Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time

doi:10.3748/wjg.v18.i48.7194

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 18, Issue 48

This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (4846)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-4) series, Tables (1-3) series.

Item

Count

PDF

329

HTML

2455

Figures (1-4)

120

Tables (1-3)

125

Sum=3029

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

1065

Download

751

Sum=1816

Dec 28, 2012 (publication date) through Apr 25, 2024

Times Cited of This Article

Times Cited (9)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Dec 28, 2012; 18(48): 7194-7200
Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7194

Table 1 Analysis of establishment of models

Group	Warm ischemia time (min)	Anhepatic phase (min)	Operative duration for biliary external drainage (min)	1-wk survival rate (%)
I	3.1 ± 0.5	18.1 ± 1.2	6.2 ± 1.2	100
II	3.0 ± 0.4	17.6 ± 1.5	7.1 ± 1.4	100
III	3.3 ± 0.3	18.5 ± 1.1	6.7 ± 1.6	90
IV	3.2 ± 0.5	17.9 ± 1.7	6.5 ± 2.0	60

Table 2 Effect of different secondary warm ischemia time on liver function (mean ± SD)

Liver function	Normal value	Group	6 h	24 h	3 d	7 d
ALT	5-40	I	165.71 ± 31.42	140.22 ± 21.08	24.30 ± 12.04	20.15 ± 8.12
(U/L)		II	356.15 ± 52.52^a	246.31 ± 22.93^a	46.43 ± 4.69^a	38.24 ± 3.52^a
		III	632.23 ± 32.28^b^c	444.32 ± 27.80^b^c	79.58 ± 17.93^a^c	56.12 ± 10.23^a^c
		IV	931.27 ± 20.21^b^d^e	801.81 ± 25.17^b^d^e	108.95 ± 11.81^b^d^e	71.26 ± 9.14^b^d^e
AST	8-40	I	855.11 ± 28.20	553.62 ± 17.66	82.02 ± 21.14	52.13 ± 17.26
(U/L)		II	1027.05 ± 42.02^a	837.31 ± 47.72^a	117.33 ± 30.86^a	84.24 ± 18.92^a
		III	1560.46 ± 68.39^b^c	1129.26 ± 137.09^b^c	173.48 ± 33.61^a^c	113.13 ± 25.72^a^c
		IV	2620.13 ± 123.68^b^d^e	2194.53 ± 297.70^b^d^e	250.10 ± 48.54^b^d^e	165.31 ± 41.26^b^d^e
AKP	47-185	I	124.52 ± 26.26	154.13 ± 32.32	121.22 ± 14.21	91.52 ± 11.56
(U/L)		II	206.54 ± 27.69^a	270.85 ± 28.48^a	189.74 ± 24.28^a	126.51 ± 16.15^a
		III	297.40 ± 34.14^b^c	363.36 ± 30.73^b^c	295.90 ± 22.04^b^c	163.26 ± 13.29^b^c
		IV	417.06 ± 10.82^b^d^e	445.81 ± 33.49^b^d^e	327.92 ± 12.36^b^c^e	227.84 ± 32.16^b^c^e
TB	0.3-1.2	I	0.82 ± 0.26	1.42 ± 0.27	0.82 ± 0.24	0.70 ± 0.21
(mg/dL)		II	1.13 ± 0.17^a	1.52 ± 0.10	0.93 ± 0.11	0.81 ± 0.16
		III	2.12 ± 0.20^a^c	2.54 ± 0.19^a^c	1.37 ± 0.20^a^c	1.04 ± 0.18^a^c
		IV	2.64 ± 0.28^b^c^e	3.43 ± 0.19^b^d^e	2.34 ± 0.27^b^c^e	1.96 ± 0.28^b^c^e
DB	0.1-0.4	I	0.83 ± 0.17	1.06 ± 0.11	0.64 ± 0.11	0.52 ± 0.21
(mg/dL)		II	1.02 ± 0.11	1.33 ± 0.12^a	0.83 ± 0.12	0.71 ± 0.16
		III	2.05 ± 0.22^a^c	2.36 ± 0.20^a^c	1.45 ± 0.22^a^c	1.32 ± 0.19^a^c
		IV	2.36 ± 0.12^b^c^e	2.75 ± 0.12^a^c^e	2.23 ± 0.20^b^d^e	2.02 ± 0.26^b^d^e

^aP < 0.05,

^bP < 0.01 vs group I;

^cP < 0.05,

^dP < 0.01 vs group II;

^eP < 0.05 vs group III. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; AKP: Alkaline phosphatase; TB: Total bilirubin; DB: Direct bilirubin.

Table 3 Effect of different timing of secondary warm ischemia on apoptosis index of bile duct epithelia and bile duct injury severity score (mean ± SD)

Group	Apoptosis index of bile duct epithelia		Bile duct injury severity score
Group	6 h	24 h	6 h	24 h
I	5.87 ± 0.50	7.13 ± 0.60	2.5 ± 0.3	2.9 ± 0.2
II	7.58 ± 0.31^a	8.96 ± 0.67^a	3.6 ± 0.3^a	3.7 ± 0.4^a
III	8.98 ± 0.69^a^c	9.90 ± 0.48^a^c	4.7 ± 0.2^a^c	5.1 ± 0.3^a^c
IV	11.99 ± 0.49^b^c^e	13.08 ± 0.65^b^d^e	6.1 ± 0.4^b^e	6.5 ± 0.2^b^c^e

^aP < 0.05,

^bP < 0.01 vs group I;

^cP < 0.05,

^dP < 0.01 vs group II;

^eP < 0.05 vs group III.

Citation: Zhu XH, Pan JP, Wu YF, Ding YT. Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time. World J Gastroenterol 2012; 18(48): 7194-7200
URL: https://www.wjgnet.com/1007-9327/full/v18/i48/7194.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i48.7194