Original Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. May 14, 2012; 18(18): 2180-2187
Published online May 14, 2012. doi: 10.3748/wjg.v18.i18.2180
Table 1 Primer sets used for quantitative reverse transcriptase polymerase chain reaction of mouse large intestine tissue
Gene nameAccession no.Product sizeDirectionSequence (5’→3’)Source
IL-6NM_031168.1107 bpForwardGCTGGTGACAACCACGGCCTThis study
ReverseAGCCTCCGACTTGTGAAGTGGT
TNF-αNM_013693.267 bpForwardTGGGACAGTGACCTGGACTGT[58]
ReverseTTCGGAAAGCCCATTTGAGT
Claudin 3NM_009902.4132 bpForwardCAGGGGCAGTCTCTGTGCGAGThis study
ReverseGCCGCTGGACCTGGGAATCAAC
OccludinNM_008756.2308 bpForwardATGTCCGGCCGATGCTCTC[58]
ReverseTTTGGCTGCTCTTGGGTCTGTAT
ZO-1NM_009386.2403 bpForwardACCCGAAACTGATGCTGTGGATAG[58]
ReverseAAATGGCCGGGCAGAACTTGTGTA
ZO-2AF113005.1106 bpForwardCCCAGCACCAAGCCACCTTTTCAThis study
ReverseTCGGTTAGGGCAGACACACTCCC
GAPDHNM_008084.2132 bpForwardAACTTTGGCATTGTGGAAGGThis study
ReverseGGATGCAGGGATGATGTTCT
Table 2 Primer sets used for quantitative polymerase chain reaction of 16S rRNA of specific bacterial species or genus
Target organismPrimer setSequence (5’ to 3’)Product sizeAnnealing temp (°C)Reference
BacteroidesBactF285GGTTCTGAGAGGAGGTCCC5361[59]
UniR338GCTGCCTCCCGTAGGAGT61
Clostridium butyricumCbut825FGTGCCGCCGCTAACGCATTAAGTAT21372[60]
Cbut1038RACCATGCACCACCTGTCTTCCTGCC72
Clostridium clostridiiformeCclos99FAATCTTGATTGACTGAGTGGCGGAC14862[60]
Cclos247RCCATCTCACACTACCGGAGTTTTTC62
Clostridium perfringensCperf165FCGCATAACGTTGAAAGATGG10461[59]
Cperf269RCCTTGGTAGGCCGTTACCC61
EnterobacteriaceaeEco1457FCATTGACGTTACCCGCAGAAGAAGC19563[60]
Eco1652RCTCTACGAGACTCAAGCTTGC63
EnterococcusEc-ssu1FGGATAACACTTGGAAACAGG11560[61]
Ec-ssu1RTCCTTGTTCTTCTCTAACAA60
EubacteriaUniF340ACTCCTACGGGAGGCAGCAGT21063[62]
UniR514ATTACCGCGGCTGCTGGC63
Faecalibacterium prausnitziiFprau223FGATGGCCTCGCGTCCGATTAG19958[60]
Fprau420RCCGAAGACCTTCTTCCTCC58
LactoccociLabF362AGCAGTAGGGAATCTTCCA31556[59]
LabR677CACCGCTACACATGGAG56
Mouse intestinal BacteriaUni516FCCAGCAGCCGCGGTAATA16158[59]
MIBR677CGCATTCCGCATACTTCTC58
Segmented filamentous bacteriaSFB736FGACGCTGAGGCATGAGAGCAT10858[59]
SFB844RGACGGCACGGATTGTTATTCA58
Ruminococcus albusRalb561FCAGGTGTGAAATTTAGGGGC24663[60]
Ralb807RGTCAGTCCCCCCACACCTAG63