Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure

Table 1 Primers used for reverse transcription-polymerase chain reaction

Gene	Primer (5’-3’)		Amplicon(bp)
	Forward primer	Reverse primer
ALB	CTTTCAAAGCATGGGCAGTAG	GCAGCAGCACGACAGAGTAA	411
GATA-4	ACCTGGGACTTGGAGGATAG	GACAAGGACATCTTGGGAAA	250
AFP	TGAGCACTGTTGCAGAGGAG	CTGAGACAGCAAGCTGAGGA	308

ALB: Albumin; AFP: α-fetoprotein.

Table 2 Changes in serum biochemical indexes at different times

	48 h after injection D-GalN	48 h after transplantation			7 d after transplantation
	All 3 groups	Encapsulated group	Unencapsulated group	PBS group	Encapsulated group	Unencapsulated group	PBS group
ALT (U/L)	3242.3 ± 2403.24	93.93 ± 63.45b	126.1 ± 54.35	245.9 ± 67.87	42.25 ± 11.86	45.07 ± 10.56	47.27 ± 11.08
AST (U/L)	4237.20 ± 1372.07	168.87 ± 89.33b	275.7 ± 52.74	439.7 ± 133.01	162.6 ± 54.29	124.52 ± 24.61	114.83 ± 16.50
TBIL (μmol/L)	5.57 ± 1.86	1.73 ± 1.01a	2.23 ± 1.98	3.50 ± 1.23	1.90 ± 0.52	2.72 ± 0.96	3.72 ± 1.18

Data are shown as means ± SD.

^aP < 0.05;

^bP < 0.01, in comparison with PBS group. TBIL: total bilirubin; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase.