Celecoxib inhibits Helicobacter pylori colonization-related factors

Table 1 Primers and probes used in real-time quantitative PCR

Gene	Primer (5’-3’)
flaA-F	ATTGGCGTGTTAGCAGAAGTGA
flaA-R	TGACTGGACCGCCACATC
flaB-F	ACATCATTGTGAGCGGTGTGA
flaB-R	GCCCCTAACCGCTCTCAAAT
ureA-F	GCTGGTGCGATTGGCTTTA
ureA-R	GGATAGCGACTTGCACATCGT
ureB-F	TCCTGATGGGACAAAACTCGTA
ureB-R	ACGGCTTTTTTGCCTTCGT
babA-F	TGCTCAGGGCAAGGGAATAA
babA-R	ATCGTGGTGGTTACGCTTTTG
sabA-F	GGTGTGCTGCAACAGACTCAA
sabA-R	CATAAGCTGTTGCGCCAAATT
alpA-F	GCACGATCGGTAGCCAGACT
alpA-R	ACACATTCCCCGCATTCAAG
alpB-F	ACGCTAAGAAACAGCCCTCAAC
alpB-R	TCATGCGTAACCCCACATCA
hpaA-F	GAGCGTGGTGGCTTTGTTAGT
hpaA-R	TCGCTAGCTGGATGGTAATTCA
hopZ -F	GCGCCGTTACTAGCATGATCA
hopZ-R	GAAATCTTTCGGCGCGTTT
16SrRNA-F	CCGCCTACGCGCTCTTTAC
16SrRNA-R	CTAACGAATAAGCACCGGCTAAC

PCR: Polymerase chain reaction.

Table 2 mRNA levels in H. pylori flagellin, urease and adhesin genes meausred by real-time quantitative PCR (mean ± SD)

Gene	Celecoxib (0.02 mmol/L)	DMSO control (1/1000)
flaA	23.08 ± 1.70a	51.08 ± 6.91
flaB	16.01 ± 0.04a	34.80 ± 7.13
ureA	19.61 ± 1.78a	7.65 ± 0.38
ureB	29.59 ± 5.31a	13.80 ± 1.63
babA	16.78 ± 0.91a	12.38 ± 0.38
sabA	49.00 ± 4.10a	22.55 ± 2.26
alpA	15.55 ± 0.78a	7.34 ± 0.20
alpB	14.07 ± 0.23a	8.95 ± 0.38
hpaA	123.98 ± 11.82a	57.15 ± 2.56
hopZ	100.25 ± 4.37a	45.54 ± 11.64

^aP < 0.05 vs DMSO control.