Celecoxib inhibits Helicobacter pylori colonization-related factors

doi:10.3748/wjg.v16.i7.846

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Feb 21, 2010; 16(7): 846-853
Published online Feb 21, 2010. doi: 10.3748/wjg.v16.i7.846

Celecoxib inhibits Helicobacter pylori colonization-related factors

Jing Wang, Wei-Hong Wang, Jiang Li, Fang-Xun Liu

Jing Wang, Wei-Hong Wang, Jiang Li, Fang-Xun Liu, Department of Gastroenterology, Peking University First Hospital, Beijing l00034, China

Author contributions: Wang J and Wang WH contributed equally to this work; Wang J performed the most parts of experiment and wrote the manuscript; Wang WH designed the study and wrote the manuscript; Li J and Liu FX offered the technical assistance.

Supported by National Natural Science Foundation of China, No. 30770981

Correspondence to: Wei-Hong Wang, Professor, Department of Gastroenterology, Peking University First Hospital, 8 Xishiku Avenue, Xicheng District, Beijing 100034, China. wangweihong@medmail.com.cn

Telephone: +86-10-83572616 Fax: +86-10-66518105

Received: November 25, 2009
Revised: December 14, 2009
Accepted: December 21, 2009
Published online: February 21, 2010

Abstract

AIM: To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism.

METHODS: After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction.

RESULTS: Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib.

CONCLUSION: Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro.

Keywords: Helicobacter pylori, Celecoxib, Colonization, Ultrastructure