Original Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Apr 14, 2009; 15(14): 1730-1737
Published online Apr 14, 2009. doi: 10.3748/wjg.15.1730
Table 1 Changes in histomorphometry of pancreatic islets of diabetic rats after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)
TreatmentIslet number islets/pancrease sectionIslet size cells/islet sectionNecrosisAtrophyCell infiltration
Normal rats3.50 ± 0.843.83 ± 0.4500.17 ± 0.410
Diabetic rats1.08 ± 0.29b1.00 ± 0.0b3.25 ± 0.45b2.08 ± 0.29a0
Bu-Zhong-Yi-Qi-Tang-treated rats1.17 ± 0.41b2.5 ± 0.55bd0.33 ± 0.52d1.17 ± 0.41ad0
Table 2 Levels of nitrite and nitrate in serum and pancreas tissue of diabetic rats after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)
TreatmentNitrite &mgr;mol/L serumNitrate &mgr;mol/L serumNitrite &mgr;mol/L per gram wet tissueNitrate &mgr;mol/L per gram wet tissue
Normal rats21.33 ± 0.2598.78 ± 49.20146.70 ± 24.7713.29 ± 10.73
Diabetic rats14.00 ± 0.19b85.76 ± 30.0895.60 ± 28.89b11.59 ± 9.54
Bu-Zhong-Yi-Qi-Tang-treated rats25.98 ± 0.74bd114.78 ± 30.09d132.82 ± 54.03d21.30 ± 18.73
Table 3 Activity of α-mannosidase and amylase in serum and pancreas tissue of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)
TreatmentSerum enzyme activities (U/mL serum)
Tissue enzyme activities (U/g wet tissue)
α-mannosidaseAmylaseα-mannosidaseAmylase
Normal rats3.60 ± 2.4328.71 ± 11.0781.78 ± 30.3419.53 ± 3.84
Diabetic rats3.17 ± 7.0717.35 ± 13.76a20.26 ± 20.42b13.15 ± 0.98a
Bu-Zhong-Yi-Qi-Tang-treated rats1.53 ± 1.7225.14 ± 8.76c44.79 ± 26.93bd17.94 ± 2.20d
Table 4 Binding of lectin to amylase in serum of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)
TreatmentConA-binding% (0-binding%)LCA-binding% (0-binding%)PNA-binding% (0-binding%)PSA-binding% (0-binding%)
Normal rats22.18 ± 42.27 (71.43)2.61 ± 6.78 (85.71)3.89 ± 9.92 (85.71)6.52 ± 14.22 (78.57)
Diabetic rats31.18 ± 43.91 (58.33)27.84 ± 44.15a (58.33)32.86 ± 41.01a (50.0)25.0 ± 45.23 (75.0)
Bu-Zhong-Yi-Qi-Tang-treated rats1.20 ± 3.62ac (87.50)16.41 ± 34.06 (75.0)1.78 ± 5.0c (87.50)9.09 ± 16.44 (62.50)
Table 5 Binding of of lectin to amylase in pancreas tissue of diabetic group after treatment with Bu-Zhong-Yi-Qi-Tang (mean ± SD)
TreatmentConA-binding% (0-binding%)LCA-binding% (0-binding%)PSA-binding% (0-binding%)
Normal rats23.53 ± 27.83 (40.0)7.06 ± 15.78 (80.0)20.0 ± 44.72 (80.0)
Diabetic rats20.0 ± 35.46 (62.5)47.30 ± 40.53a (12.5)14.87 ± 27.68 (75.0)
Bu-Zhong-Yi-Qi-Tang-treated rats16.82 ± 31.18 (50.0)35.91 ± 37.08a (30.0)22.0 ± 41.46 (50.0)
Table 6 Correlation between the number, size, necrosis and atrophy of islets and levels of α-mannosidase, amylase, nitrite and nitrate and binding of lectin to amylase
Biochemistry indexnIslet numberIslet sizeNecrosisAtrophy
Serum nitrite500.404b0.736b-0.319b-0.866b
Serum nitrate500.1810.194-0.126-0.245a
Pancreas tissue nitrite500. 30a0.456b-0.222-0.480b
Pancreas tissue nitrate500.1010.157-0.041-0.317b
LCA-binding of amylase in serum61-0.254a-0.290a0.0770.228a
ConA-binding of amylase in serum61-0.048-0.10-0.011-0.016
PNA-binding of amylase in serum61-0.114-0.261a0.283a0.041
PSA-binding of amylase in serum61-0.143-0.26a0.1650.153
LCA-binding of amylase in pancreas tissue43-0.442b-0.253a0.1740.183
ConA-binding of amylase in pancreas tissue43-0.117-0.095-0.1920.232
PSA-binding of amylase in pancreas tissue43-0.095-0.037-0.288a0.106
The activity of α-mannosidase in serum610.0670.0500.026-0.108
The activity of α-mannosidase in pancreas tissue430.638b0.747b-0.508b-0.714b
The activity of amylase in serum610.1580.365b-0.215a-0.310b
The activity of amylase in pancreas tissue430.428b0.639b-0.314a-0.631b