A simple and rapid method for extracting bacterial DNA from intestinal microflora for ERIC-PCR detection

Table 1 DNA yields and purity obtained by six DNA extraction methods (&mgr;g DNA/g sample)

Samples	T-	C-	UW-	SDS-	SDSS-	FDK-
	method	method	method	method	method	method
Yield	601.52	1301.52	586.03	1710.95	1820.12	1326.07
Purity (A260/280)	1.58	1.84	1.6	1.54	1.49	1.82

Table 2 Cs matrix values of ERIC-PCR fingerprint of the samples extracted by different methods (%)

Samples	T-	C-	UW-	SDS-	SDSS-	FDK-
	method	method	method	method	method	method
C-method	25	-	25	0	0	92.1
UW-method	100	25	-	0	0	25
SDS-method	0	0	0	-	100	0
SDSS-method	0	0	0	100	-	0
FDK-method	25	92.1	25	25	25	-

Table 3 Cs matrix values of ERIC-PCR fingerprint of the samples extracted by C-method and FDK-methods (%)

Samples	C-method (1)	C-method (2)	FDK-method (1)	FDK-method (2)
C-method (2)	100.0		92.1	92.1
FDK-method (1)	92.1	92.1		100.0
FDK-method (2)	92.1	92.1	100.0