Ephrin-B reverse signaling induces expression of wound healing associated genes in IEC-6 intestinal epithelial cells

Table 1 Selection of significantly regulated genes (fold change >2) upon stimulation of ephrin-B pathways in IEC-6 cells

Gene bank accession	Title	Function	FC (30 min1)	FC (120 min1)
gb:AA944459	Dynein, cytoplasmic, light intermediate chain 1	Migration/polarity/Wound edge	-2	NC
gb:AA875047	JQ0866 T-complex protein 1 – rat	Chaperonin/protein folding	-2	NC
gb:AI231350	Transcribed sequences	Unknown	-2	NC
gb:AI071071	Transcribed sequences	Unknown	-2	NC
gb:BE097926	Transcribed sequences	Unknown	-2.1	NC
gb:AA925921	ELV4_RAT ELAV-like protein 4	Binds to the AU-rich element in c-fos	-2.1	NC
		and interleukin-3/mRNA degradation
gb:AW144020	Transcribed sequences	Unknown	-2.1	NC
gb:U30789.1	Upregulated by 1,25-dihydroxyvitamin D-3	Unknown	-2.3	-2.8
gb:AI230596	Importin alpha S1	Nuclear transport/DNA binding	-2.3	NC
gb:BF389856	Beta-galactosidase, alpha peptide	Metabolism	-2.3	NC
gb:NM_012903.1	Acidic nuc. phosphoprotein 32 family, member A	Apoptosis	-3.2	NC
gb:BF415939	c-fos	Wound healing	16	NC
gb:NM_053633.1	Early growth response 2	Apoptosis/EGR2 induces apoptosis	7.5	NC
gb:NM_024360.1	HES-1/hairy and enhancer of split 1 (Drosophila)	Notch pathway target/neurite	4.9	NC
		outgrowth/morphogenesis
gb:NM_012551.1	Early growth response 1	Apoptosis/EGR2 induces apoptosis	4.6	NC
gb:U03389.1	Cox-2/prostaglandin-endoperoxide synthase 2	Epithelial wound healing	4.6	4
gb:BE110108	MAP kinase phosphatase-1/protein	Signaling/stress	3.5	NC
	tyrosine phosphatase
gb:NM_021836.1	Jun B proto-oncogene	Transcription factor/wound healing cornea	3	NC
gb:AI178746	Transcribed sequences	Unknown	3	NC
gb:NM_012620.1	PAI-1/serine (or cysteine) proteinase	Affect wound healing by regulating the	2.8	NC
	inhibitor, member 1	fibrinolytic environment
gb:AI710284	Beta-galactosidase, alpha peptide	Unknown	2.8	3.2
gb:BF420059	Immediate-early protein pip92	Unknown	2.6	NC
gb:BI288619	c-jun homolog/v-jun sarcoma virus 17	Transcription factor/wound repair/epithelial	2.6	NC
	oncogene homolog (avian)	migration
gb:BI298889	RBBP-2	Transcription factor/cell cycle inhibition	2.6	NC
gb:NM_031642.1	Core promoter element binding protein	Unknown	2.5	NC
gb:AB025017	Zinc finger protein 36	Unknown	2.5	NC
gb:AI599423	DNA-damage-inducible protein GADD45 gamma	Cell cycle control	2.5	NC
gb:U02553.1	MAP kinase phosphatase-1/protein	Signaling/stress	2.3	NC
	tyrosine phosphatase
gb:L81174.1	Ankyrin-like repeat protein	Endothelial activation	2.1	NC
gb:NM_017206.1	Solute carrier family 6, member 6	Protects IECs from osmotic stress	2.1	NC
gb:BM383427	Gp130/Interleukin 6 signal transducer	Host defense/tight junction loosening/	2.1	2.3
		proinflammatory lipid production
gb:AI179464	Rattus norvegicus RM1 mRNA, partial sequence	Unknown	2.1	3.2
gb:NM_031530.1	MCP-1/small inducible cytokine A2	Monocyte chemotaxis/candidate locus for exp. EM	2	NC
gb:NM_022858.1	HNF-3/forkhead homolog-1	Transcription factor/development	2	2.6
gb:AA850780	Transcribed sequences	Unknown	2	NC
gb:NM_012940.1	Cytochrome P450, subfamily 1B, polypeptide 1	Detoxification	2	5.3
gb:NM_013057.1	Coagulation factor 3	Blood coagulation	2	NC
gb:BI284349	Myeloid differentiation primary response gene 116	Unknown	2	NC
gb:AI176519	DIF-2/lipid responsive gene/Immediate	Differentiation	2	NC
	early response 3 protein
gb:AI169756	G33_rat gene 33 polypeptide	Migration/signaling/adapter protein	2	NC
	binds GTP-Cdc42/activates SAPK/JNK
gb:BI288619	c-jun homolog/v-jun sarcoma virus	Transcription factor/wound repair/epithelial	2	NC
	17 oncogene homolog (avian)	migration
gb:AA800192	Rattus norvegicus endogenous	Unknown	2	NC
	retrovirus mRNA, partial sequence
gb:AW533292	Similarity to protein ref: NP_073600.1 (H. sapiens)	Unknown	2	NC
gb:BF392456	Rattus norvegicus non-erythrocyte	Unknown	2	NC
	beta-spectrin mRNA, partial cds

¹IEC-6 cells were treated with 0.5 mg/mL (- 3.3 nmol/L) rat recombinant EphB1-Fc and RNA was harvested after 30 and 120 min. FC: fold changes; NC: no changes.