Mitochondrial DNA sequence analysis of two mouse hepatocarcinoma cell lines

Table 1 Primer sequence and length of amplified fragments.

Amplified fragment	Length (bp)	Location	Primer sequence
D-loop	1100	L strand (15 294-15 320)	5’-TAAACATTACTCTGGTCTTGTAAACC-3’
		H strand (98-72)	5’-ATTAATAAGGCCAGGACCAAACCT-3’
tRNAMet+Glu+Ile	1126	L strand (3 401-3 419)	5’-CGGCCCATTCGCGTTATTC-3’
		H strand (4 527-4 508)	5’-AGGTTGAGTAGAGTGAGGGA-3’
ND3 fragment	534	L strand (9 364-9 385)	5’-ACGTCTCCATTTATTGATGAGG-3’
		H strand (9 897-9 876)	5’-GAGGTTGAAGAAGGTAGATGGC-3’
D-loop 3’ end fragment	437	L strand (15 950-15 968)	5’-AGGCATGAAAGGACAGCAC-3’
		H strand (91-73)	5’-ATAAGGCCAGGACCAAACC-3’

Table 2 Mitochondrial tRNA^Met+Glu+Ile, ND3 and D-loop restriction patterns.

Enzyme	D-loop (1 100 bp)		tRNAMet+Glu+Ile (1 126 bp)		ND3 (534 bp)
	Site	FL	Site	FL	Site	FL
Hae III	4	456445122, ...	4	470290136...	1	392142
Apa I	1	655445	1	658469	0	534
Alu I	4	545269102, ...	3	665318135...	1	310224
BamH I	0	1100	2	710252164	0	534
Dra I	0	1100	1	634493	1	45183
Stu I	0	1100	2	605297225	1	391143
Nde II	2	84 511 837	0	1 126	0	534
Rsa I	6	720178125...	4	392259228...	2	35310378
Xba I	1	680420	0	1126	0	524