Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells

Table 1 Oligonucleotide primers for RT-PCR (Sangon, Shanghai, China).

Gene	cDNA primer sequence	Annealing temperature (°C)	Product size (bp)
TGF-b1	Forward: 5’-GCGACTCGCCAGAGTGTTAT-3’
	Reverse: 5’-ATAGTTGGTGTCCAGGGCTCG-3’	61	284
TbRI	Forward: 5’-ACGGCGTTACAGTGTTCTG-3’
	Reverse: 5’-CCACACCGTCTATATCTGG-3’	57	358
TbRII	Forward: 5’-AGCAACTGCAGCATCACCTC-3’
	Reverse: 5’-ACTACAGACTCTTCTACAGG-3’	57	688
Smad2	Forward: 5’-ATCCTAACAGAACTTCCGCC-3’
	Reverse: 5’-GAGTCGTTTTTGAAGGGGTG-3’	57	489
Smad3	Forward: 5’-GGAGGGCAGGCTTGGGGAAAATG-3’
	Reverse: 5’-CCCCTCCCCCGGCCACCACATTAT-3’	57	284
Smad4	Forward: 5’-AAGGTGAAGGTGATGTTTG-3’
	Reverse: 5’-CTCGATAAGGTGGATGACTA-3’	56	264
Smad7	Forward: 5’-GTGGGGAGGCTCTACTGTGTC-3’
	Reverse: 5’-CAGCTTTCGGAACTACCTCTTTGG-3’	57	294
p15	Forward: 5’-TTCTTTAAATGGCTCCACCTGCCT-3’
	Reverse: 5’-AACCGTCGGAAGTAGCTTAATCCA-3’	60	436
p21	Forward: 5’-AGTGGACAGCGAGCAGCTGA-3’
	Reverse: 5’-ATCTTTAGACAGTACGACCAGAC-3’	58	380

Table 2 Cell cycle alterations of BGC-823 cells treated with TGF-β1.

Time of TGF-b1treatment (h)	Cell cycle (%)
	G0-G1	S	G2-M	Apoptosis
0	52.72	32.39	14.89	0.34
24	57.07	33.82	9.11	6.61
48	60.2	29.54	10.26	9.86