Significance of vascular endothelial growth factor expression and its correlation with inducible nitric oxide synthase in gastric cancer

Table 1 Expression of VEGF₁₆₅mRNA in gastric cancer and non-cancerous mucosa by RT-PCR

No.	Sex	Age (yr)	Histological type	Depth of invasion	Lympho node metastasis	Stage of TNM	VEGFmRNA
							T	N
1	M	67	PD	Musclaris	-	I	1.286	0.782
2	M	67	WD	Extraserosa	+	III	1.568	1.086
3	F	41	PD	Serosa	+	III	1.624	0.925
4	M	54	PD	Extraserosa	+	IV	0.815	0.729
5	M	35	PD	Extraserosa	+	III	1.204	0.627
6	M	50	MD	Extraserosa	+	III	1.111	0.335
7	F	34	WD	Extraserosa	+	III	1.347	0.845
8	M	46	MD	Serosa	+	III	0.785	0.701
9	M	60	WD	Submucosae	-	I	0.833	0.807
10	F	70	WD	Serosa	-	I	0.627	0.525
11	M	62	WD	Serosa	+	IV	0.850	0.646
12	F	31	WD	Mucosae	-	I	0.815	1.136
13	M	55	MD	Extraserosa	+	IV	1.545	0.872
14	M	70	WD	Serosa	+	II	0.765	0.577
15	M	64	MD	Serosa	-	III	1.082	0.842
16	M	66	MD	Serosa	+	III	1.069	0.422
17	M	77	PD	Serosa	+	IV	1.383	10.28
18	M	40	PD	Serosa	+	II	1.156	1.381
19	F	50	PD	Serosa	+	II	0.989	0.874
20	M	52	PD	Serosa	+	III	1.895	0.356
21	F	38	PD	Extraserosa	+	III	1.667	0.759
22	F	68	WD	Mucosae	-	I	0.884	0.682
23	F	53	WD	Extraserosa	+	III	1.456	0.682
24	M	61	MD	Extraserosa	+	III	1.574	0.098
25	M	43	PD	Serosa	-	I	1.198	0.429
26	M	43	PD	Serosa	+	III	0.368	0.994
27	M	57	PD	Serosa	+	II	1.052	0.661
28	F	44	PD	Mucosae	-	I	0.952	0.793
29	M	71	WD	Extraserosa	-	II	0.750	0.728
30	M	77	PD	Extraserosa	+	IV	1.375	10105
31	F	50	PD	Mucosae	-	I	0.842	0.794
Mean value							1.125	0.760

Table 2 Relationship between expression of VEGF₁₆₅mRNA and clinical pathological features in gastric cancer tissue

Variable	No.	Expression of VEGF (mean ± SD)
Age (yr)			NS
< 55	16	1.139 ± 0.393
> 55	15	1.139 ± 0.393
Sizes of tumor			NS
2 cm	2	0.993 ± 0.271
2-3 cm	6
> 3 cm	23	1.171 ± 0.375
Histological type			NS
Well differentiated	10	0.979 ± 0.339
Moderate or poorly differentiated	21	1.1937 ± 0.350
Lymph node metastasis			0.03
Positive	21	1.219 ± 0.377
Negative	10	0.927 ± 0.205
Stage of UICC			0.009
I	8	0.934 ± 0.194
II	5
III	13	1.262 ± 0.386
IV	5

NS: Not significant.

Table 3 Relationship between level of VEGF₁₆₅mRNA expres-sion and lymph node metastasis

Variable	Lymph node metastasis
	Positive cases	Negative cases	Total rate	Metastasis
Over-expression	15	3	18	83.3%
Low-expression	6	7	13	46.2%
Total	19	10	31

χ² = 4.775, P = 0.02.

Table 4 Relationship between level of VEGF₁₆₅mRNA expres-sion and stages of UICC

Variable	Stages of UICC		Total	Ratio of III + IV
	I + II	III + IV
Over-expression	4	14	18	77.8%
Low-expression	9	4	13	30.8%
Total	13	18	31

χ² = 6.85, P = 0.009.

Table 5 Association with immunoreactive grades of VEGF protein and iNOS in gastric cancer

iNOS (N)	VEGF (N)
	-	+	++	+++	Total
-	14	12	6	3	35
+	5	9	8	2	24
++	2	5	11	1	19
+++	0	2	3	2	7
Total	21	28	28	8	85

χ² = 5.615, P = 0.132.