Preparation and characteristics of DNA-nanoparticles targeting to hepatocarcinoma cells

Table 1 Distribution (%) of ³²P-DNA in mice after intravenous administration of ³²P-DNA (n = 5)

Time	Heart	Liver	Spleen	Lung	Kidney	Blood
5 min	3.14 ± 0.60	36.67 ± 2.65	3.56 ± 1.58	16.32 ± 0.79	23.52 ± 1.48	16.79 ± 1.62
15 min	3.15 ± 0.97	41.26 ± 2.89	5.25 ± 0.86	14.16 ± 0.99	20.61 ± 1.62	15.57 ± 1.58
30 min	6.94 ± 1.57	52.59 ± 4.02	10.46 ± 0.75	7.95 ± 0.58	13.10 ± 0.85	2.95 ± 0.65
1 h	6.11 ± 1.95	54.62 ± 3.12	14.85 ± 1.23	7.82 ± 0.67	13.50 ± 0.94	3.10 ± 0.78
2 h	4.56 ± 1.01	51.34 ± 2.88	14.93 ± 0.68	9.65 ± 1.41	15.03 ± 1.34	4.49 ± 0.28
6 h	5.12 ± 0.79	48.66 ± 1.87	13.15 ± 0.84	9.66 ± 1.27	18.26 ± 1.58	5.15 ± 1.67
12 h	5.48 ± 0.99	44.52 ± 2.02	11.32 ± 1.33	11.36 ± 1.34	21.83 ± 2.54	5.49 ± 0.58
24 h	3.93 ± 0.65	43.31 ± 1.67	11.48 ± 1.11	10.12 ± 0.64	25.01 ± 3.12	6.16 ± 0.94
48 h	3.43 ± 0.84	36.75 ± 1.65	11.56 ± 0.82	13.36 ± 1.60	28.52 ± 2.15	6.38 ± 0.83
72 h	4.52 ± 0.77	34.48 ± 1.85	12.03 ± 1.73	13.88 ± 1.63	28.31 ± 3.01	6.78 ± 0.91

Table 2 Distribution (%) of ³²P-DNA in mice after intravenous administration of ³²P-DNA-PLGA nanoparticles (n = 5)

Time	Heart	Liver	Spleen	Lung	Kidney	Blood
5 min	2.22 ± 0.51	57.32 ± 2.36	2.96 ± 0.69	8.29 ± 1.33	16.58 ± 2.02	12.63 ± 1.24
15 min	3.52 ± 0.64	69.69 ± 3.32	3.19 ± 0.58	5.77 ± 0.55	13.87 ± 0.99	3.96 ± 0.36
30 min	2.38 ± 0.67	73.37 ± 3.62	7.55 ± 1.03	3.64 ± 0.41	11.28 ± 1.28	1.78 ± 0.65
1 h	2.08 ± 0.54	80.14 ± 4.56	3.37 ± 0.67	3.08 ± 0.62	9.34 ± 0.68	1.99 ± 0.54
2 h	2.59 ± 0.28	78.45 ± 4.02	3.73 ± 0.76	5.35 ± 0.58	8.36 ± 0.94	1.47 ± 0.23
6 h	3.18 ± 0.60	75.03 ± 3.69	7.38 ± 1.03	3.74 ± 0.39	9.61 ± 0.96	1.06 ± 0.24
12 h	2.21 ± 0.51	69.34 ± 3.96	6.17 ± 1.24	7.88 ± 0.64	10.17 ± 1.04	4.22 ± 0.32
24 h	1.94 ± 0.32	65.59 ± 3.25	6.46 ± 1.04	7.95 ± 0.57	12.10 ± 1.23	5.96 ± 0.59
48 h	2.21 ± 0.29	61.18 ± 2.69	7.01 ± 0.48	9.27 ± 0.71	12.87 ± 0.86	6.46 ± 0.86
72 h	2.51 ± 0.37	60.56 ± 2.98	6.45 ± 0.69	9.98 ± 0.65	13.54 ± 1.11	6.96 ± 0.75

Table 3 Inhibition ratio of different concentrations of GCV on SMMC-7721 and Chang liver cells

GCV (µg/mL)	SMMC-7721		Chang liver
	DNA	NP	DNA	NP
1	2.0	6.6a	1.2	3.7
10	3.0	10.0a	2.8	5.8

^aP < 0.05 vs Chang liver NP group; NP: nanoparticles.

Table 4 Mean fluorescence intensity in SMMC-7721 and Chang liver cells

Cell	MFI
	DNA	NP
SMMC-7721	0.6 ± 0.1a	2.1 ± 0.3c
Chang liver	0.6 ± 0.2	0.7 ± 0.2

^aP < 0.05 vs NP group;

^cP < 0.05 vs Chang liver NP group; NP: nanoparticles.