Liver Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. Feb 15, 2004; 10(4): 509-513
Published online Feb 15, 2004. doi: 10.3748/wjg.v10.i4.509
Table 1 Primer sequences and PCR conditions used for synthesis of amplicons applied as probes in Northern blot and RT-PCR
Genes namePrimer (5’-3’)PCR fragment size (bp)Annealing temperatureNumber of cycles
Pir51F GTGGAAGATGATGTTGGTGGTG5275832
R AAGGCGGAGACTCTGATTGG
RbAp48F GAACTGCCTTTCTTTCAATC8265830
R ATGGCTCAGACACCTACCTC
Beclin 1F CTTACCACAGCCCAGGCGAAAC8145830
R GCCAGAGCATGGAGCAGCAA
Aldolase bF GCCACTCTCAACCTCAATGC4235832
R TCTCCTTCCCAACCTACCAC
β-actinF TGACGGGGTCACCCACACTGTGCC6666025
R CTTAGAAGCATTGCGGTGGACGATG
Table 2 Classification of number of known functioning genes differentially expressed (> 4 folds) in HCC
Gene functionsNumber of down-regulated genes in HCCNumber of up-regulated genes in HCC
Cell division1714
Cell, organism defense3924
Metabolic enzymes, transporters ion channels179
Nuclear proteins (transcription factors, DNA processing enzymes)2120
Cell structure, extracellular matrix106
Cell signalling, communication3726
EST4153
Other genes84102
Table 3 Genes showing differential expression levels in liver cancerous tissue and adjacent normal liver tissue
Gene NameGenBank access numberDensity in filter for tumor liverDensity in filter for normal liverFold change by microarrayFold change by Northern blot
Beclin 1AF077301697109999
RbAp48X7426214029.909999
aldolase bXM_005563276526589-9.65-4.7
Pir51NM_0064798654.61323.226.85.7