Alteration of p53 and p21 during hepatocarcinogenesis in tree shrews

Table 1 Status of p53 gene from liver and tumor tissues of tree shrews

Animal (Groups)	Treatment		Tissues	Grade of tumors	Status of p53 gene	Change of nucleic acid
	HBV	AFB1
5201 (C)	-	+	liver	-	wt	-
5207 (C)	-	+	tumor	I	wt	-
5063 (A)	+	+	tumor	II	mt (78)	GCA-GCG
5067 (A)	+	+	tumor	II	wt	-
5172 (A)	+	+	tumor	III	mt (13)	CCT-CTT
5173 (A)	+	+	tumor	II	mt (275)	TGT-CGT
1 (D)	-	-	liver	-	wt	-
2 (D)	-	-	liver	-	wt	-

Wt: Wild type; mt: Mutation.

Table 2 Expression of p21 in normal liver and liver tumor tissues of tree shrews

Group	No. of animals (n)	incidence of HCC (%)	p21+		p21-
			Animal (n)	Incidence of HCC (%)	Animal (n)	Incidence of HCC (%)
A	39	23 (58.97)bd	16	16 (100.0)af	23	7 (30.43)fj
B	28	1 (3.57)	2	0	26	1 (3.84)
C	29	6 (20.68)	7	4 (54.14)h	22	2 (9.09)
D	20	0	0		20	0

^bP < 0.01 vs Group C;

^dP < 0.001 vs Group B;

^fP < 0.001, p21+ vs p21- in Group A;

^hP < 0.01, p21+ vs p21- in Group C;

^aP < 0.05 vs Group C in p21+;

^jP < 0.01 vs Group B in p21-.

Table 3 Relationship between p21 expression and HCC de-velopment

Group	Animal (n)	HCC (n)	Rate of HCC (%)
p21-positive	25	20	80.0b
p21-negative	91	10	10.99

^bP < 0.001 vs p21-negative.