Establishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA

Table 1 Results of serum samples from different genera detected by alkaline phosphatase direct-labeled probe

Genera	Samples (n)	DHBV DNA positive samples (n)	DHBV DNA negative samples (n)
Duck serum	20	10	10
Human serum	20	0	20
Rat serum	20	0	20

Table 2 Comparison of the results of 100 serum samples detected with different probes

Probe	Positive samples (n)	Negative samples (n)
Digoxigenin-labeled probe	60	40
Alkaline phosphatase	60	40
direct-labeled probe

Table 3 Correlation analyses of DHBV DNA quantitative results of 70 serum samples detected by two methods

Serum samples (n)	qPCR (mean ± SD) (copy/mL)	Dot-blot hybridization using digoxigenin-labeled probe (mean ± SD) (volume, mm3)
10	(2.37 ± 1.70) × 102	-
10	(2.44 ± 1.71) × 103	1155 ± 25
10	(4.47 ± 1.96) × 104	1241 ± 32
10	(5.02 ± 2.14) × 105	1353 ± 27
10	(1.83 ± 1.03) × 106	1428 ± 25
10	(4.81 ± 2.61) × 107	1550 ± 40
10	(5.16 ± 2.72) × 108	1748 ± 118