Antisense imaging of colon cancer-bearing nude mice with liposome-entrapped 99m-technetium-labeled antisense oligonucleotides of c-myc mRNA

Table 1 Course of biodistribution of liposome-entrapped ^99mTc-labeled antisense oligonucleotides in tumor-bearing nude mice (mean ± SD, percent of injected dose, n = 5 mice for each time)

Tissue	0.5 h	1 h	2 h	4 h
Liver	8.78 ± 0.63	9.16 ± 1.14	7.92 ± 0.38c	8.97 ± 0.12c
Spleen	6.78 ± 0.37c	8.86 ± 0.60	7.37 ± 0.64c	8.02 ± 0.23c
Kidney	4.89 ± 0.67a	2.90 ± 1.19a	3.07 ± 0.18ac	0.47 ± 0.02ac
Lung	10.23 ± 1.02	13.37 ± 0.84	12.21 ± 0.42a	10.20 ± 0.50c
Heart	7.18 ± 0.13	8.78 ± 1.01	7.25 ± 0.18	5.28 ± 0.45ac
Blood	5.51 ± 0.24a	4.55 ± 0.15	2.81 ± 0.11ac	2.61 ± 0.06ac
Bone	1.84 ± 0.64a	3.14 ± 1.04a	1.43 ± 0.24ac	1.02 ± 0.32ac
Muscle	2.27 ± 0.37a	1.36 ± 0.60ac	1.69 ± 0.91ac	2.85 ± 0.26a
Stomach	12.45 ± 0.62a	13.77 ± 0.38	10.63 ± 0.35	6.70 ± 0.44ac
Intestines	4.54 ± 0.42a	6.68 ± 4.14	7.76 ± 0.34c	8.44 ± 0.63c
Brain	2.24 ± 0.42a	2.16 ± 2.10ac	0.68 ± 0.06ac	0.52 ± 0.06ac
Tumor	6.12 ± 0.31a	8.09 ± 0.86	13.46 ± 0.20a	4.58 ± 0.45a

^aP < 0.05 vs that of liver tissue

^cP < 0.05 vs that of tumor tissue.

Table 2 Ratios of the radioactive counts in tumor to that in the contralateral equivalent region of abdomen (mean ± SD, n = 5 for each time point)

Time point (h)	Ratios
1.0	1.2266 ± 0.1259
1.5	1.5597 ± 0.0190
2.0	1.7332 ± 0.2537
4.0	1.0182 ± 0.0495a
6.0	1.0199 ± 0.0131a

^aP < 0.05 vs ratio of 2.0 h.