Liver Cancer
Copyright ©The Author(s) 2004.
World J Gastroenterol. Jul 15, 2004; 10(14): 2034-2038
Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2034
Table 1 PCR amplification program
GeneTemperature, duration of denaturation, annealing, extensionPrimer sequenceProduct size (bp)
MAGE-194 °C, 45 sf: 5’-CGGCCGAAGGAACCTGACCCAG-3’421
65 °C, 45 sr: 5’-GCTGGAACCCTCACTGGGTTGCC-3’
72 °C, 45 s
SSX-194 °C, 60 sf: 5’-CTAAAGCATCAGAGAAGAGAAGC-3’422
57 °C, 60 sr: 5’-AGATCTCTTATTAATCTTCTCAGAAA- 3’
72 °C, 60 s
CTp1194 °C, 45 sf: 5’-CTGCCCCAGACATTGAAGAA-3’297
57 °C, 60 sr: 5’-TCCATGAATTCCTCCTCCTC-3’
72 °C, 90 s
HCA58794 °C, 30 sf: 5’-AGGCGCGAATCA AGTTAG-3’238
60 °C, 30 sr: 5’-CTCCTCTGCTGTGCTGAC-3’
72 °C, 30 s
Β2-MG94 °C, 45 sf: 5’-CTCGCGCTACTCTCTCTTTCTGG -3’335
55 °C, 45 sr: 5’-GCTTACATGTCTCGATCCCACTTA-3’
72 °C, 45 s
Table 2 Expression of CT antigens and clinico pathological indicators
Groupn1MAGE-1
SSX-1
CTp11
HCA587
CT2(+)
+-+-+-+-+-
Total number1057926762966395946987
Gender
Male907020662457334842855
Female159610596114132
Tumor diameter
≤ 5 cm26188161014121511224
> 5 cm796118601952274435763
TNM stage
I + II432914311227162221394
III + IV625012451739233725593
Hepatitis B virus
HBV (+)987424712762365543917
HBV (-)75252434370
Hepatitis C virus
HCV (+)55041414150
HCV (-)1007426722862385545937
AFP (ng/mL)
< 4035287221320151817323
≥ 40705119541646244129664
Differentiation
Well1813512612699162
Moderately614417441739223427583
Poorly2622420615111610242