Expression of subtypes of somatostatin receptors in hepatic stellate cells

Table 1 Primer sequence and expected PCR product length

Primer	designation	Sequence	Product length
SSTR1	upstream	5’-CAC GCA CCG CAG CCA ACA-3’	390 bp
	downstream	5’-GGA AGC CGT AGA GTA TGG GGT T-3’
SSTR2	upstream	5’-CCG GAG CAA CCA GTG GGG-3’	390 bp
	downstream	5’-GCG TAC AGG ATG GGG TTG GC-3’
SSTR3	upstream	5’- CCC GGG GCA TGA GCA CGT-3’	415 bp
	downstream	5’-AAG CCG TAG AGG ATG GGG TTT GC-3’
SSTR4	upstream	5’-TCG TGG GGG TGA GGC AGT AG-3’	365 bp
	downstream	5’-CAT AGA GAA TCG GGT TGG CAC AG-3’
SSTR5	upstream	5’-ATG GAG CCC CTC TCT CTG G-3’	250 bp
	downstream	5’-CGT CAG CCA CGG CCA GGT T-3’
β-actin	upstream	5’-TGG GAC GAT ATG GAG AAG AT–3’	522 bp
	downstream	5’-ATT GCC GAT AGT GAT GAC CT –3’

Table 2 Expression of SSTRs mRNA in quantity (mean ± SD)

Subtypes	n	Expression of mRNA
SSTR2	3	0.8213 ± 0.1210
SSTR3	3	0.2041 ± 0.1662b
SSTR5	3	0.1457 ± 0.1981d

^bP < 0.01 vs SSTR2,

^dP < 0.01 vs SSTR2.