Brief Reports
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 1998; 4(3): 273-274
Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.273
Effects of Yibei multi-active elements on mesenteric microcirculation in rats
Bo-Qin Shao, Yi-Ju Shi, Sai Liu, Jian Zhang, Jin-Tai Guo
Bo-Qin Shao, Sai Liu, Jian Zhang, Jin-Tai Guo, Department of Pharmacology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Yi-Ju Shi, Research Laboratory of Microcirculation, Taishan Medical College, Taishan 271000, Shandong Province, China
Bo-Qin Shao, male, born on January 23, 1944, in Qingdao, Shandong Province, graduated from Qingdao Medical College in 1969, now associated professor of pharmacology, Vice Director of Clinical Pharmacological Institute, engaged in clinical pharmacology and antiaging drug studies, having 24 papers and 7 books published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Prof. Bo-Qin Shao, Department of Pharmacology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China
Telephone: +86-532-2017880
Received: November 26, 1997
Revised: January 4, 1998
Accepted: February 2, 1998
Published online: June 15, 1998

Abstract
Key Words: microcirculation, mesentery, Yibei multi-active elements (YBMAE), blood flow velocity



TEXT

Mytilus edulis linnaeus (Yibei) belongs to mytilide, gill lamella, and mollusc. There are rich resources in Bohai and Huanghai of China. Yibei multi-active elements (YBMAE)[1] come from mytilus edulis linnaeus containing taurine, EPA, Zn, Ci, Ferris, etc. Its preparation and composition were described previously[2]. This paper aims at studying the effect of YBMAE on the mesenteric microcirculation in rats.

MATERIALS AND METHODS
Materials

YBMAE was provided by the Department of Pharmacology, Medical College of Qingdao University. Sterile amniotic fluid and Panax Notoginsenosidum (PNS) were obtained from Taishan Medical College. Wistar rats were purchased from the Animal Center of Shandong Medical University. The microcirculation monitoring system was product of Xuzhou Optic Instrument Factory, China.

Methods

Thirty Wistar rats (male or female, weighing 300 g ± 50 g) were divided into 5 groups: YBMAE group I (1.2 g/kg), group II (3 g/kg), group III (6 g/kg), PNS group (40 mg/kg) and control group (with saline). Each group consisted of 6 rats. The drugs were givenig qd for 28 d[3-5]. Thirty minutes after the last administration of drugs, the rats were anaesthetized with vinbarbitol 50 mg/kg ip. A 2-cm incision was made on the abdominal wall. The blood color, flow velocity and vessel wall clarity of tertiary blood vessels were observed, meanwhile blood flow and flow velocity were monitored with microcirculation monitoring system. Sterile amniotic fluid (1 mL/kg) was given intravenously. Theabove-mentioned indexes were monitored and video recorded immediately 10 and 30 min after amniotic fluid injection.

Statistical analysis Student’s t test was used for the statistical study.

RESULTS
Effect of YBMAE on blood flow of Wistar rat mesenteric microcirculation

Immediately, 10 min and 30 min after amniotic fluid injection, blood flow of the control group was decreased significantly (P < 0.05, P < 0.01, P < 0.01), while the blood flow of YBMAE groups I and II showed no obvious changes compared with that before amniotic fluid injection, but more significant changes than that of the control groups (P < 0.05, P < 0.01). In YBMAE group III, the blood flow at 10 min was lower than that before amniotic fluid injection (P < 0.05), significantly higher at 30 min than the control group (P < 0.01). In PNS group, the blood flow had no obvious changes immediately and 10min after amniotic fluid injection, and increased at 30 min (P < 0.01), being significantly different compared with the control group (P < 0.05, P < 0.01) (Table 1).

Table 1 Effect of the drugs on blood flow (μm3·s-1) of mesenteric microcirculation in rats (x-±s).
GroupsDosage (g/kg)nBefore amniotic fluid injectionAfter amniotic fluid injection
Immediately10 min30 min
NS6616 ± 910.79432 ± 132.68a358 ± 64.32b348 ± 84.30b
YBMAE1.26576 ± 67.41545 ± 105.47593 ± 70.63d560 ± 57.12d
3.06560 ± 57.88450 ± 86.49561 ± 77.96c588 ± 50.75d
6.06733 ± 100.76602 ± 99.26578 ± 93.03ac665 ± 73.12d
PNS0.046586 ± 60.58616 ± 67.34c638 ± 55.11d766 ± 60.40bd
Effect of YBMAE on blood flow velocity of rat mesenteric microcirculation

The blood flow velocity was greatly decreased immediately, 10 min and 30 min after amniotic fluid injection in the control group (P < 0.05, P < 0.01), while in the YBMAE group I, there was little change in the blood flow velocity, but greater than that of the control group (P < 0.05, P < 0.01). In YBMAE group II, right after amniotic injection, the velocity was significantly decreased (P < 0.05), but still faster than that of the control group. At 10 min, 30 min, the velocity was increased, but not faster than that before amniotic injection. In YBMAE group III, the velocity was decreased immediately and 10 min after injection (P < 0.05), and increased at 30 min, which was significantly faster than that of the control group at any time (P < 0.05, 0.01). In PNS group, the velocity had no obvious changes after injection, but significantly faster than the control group (P < 0.05) (Table 2).

Table 2 Effect of the drugs on the blood flow velocity of rat mesenteric microcirculation (x-±s).
GroupsDosage (g/kg)nBefore amniotic fluid injectionAfter amniotic fluid injection
Immediately10 min30 min
NS60.51 ± 0.100.35 ± 0.05a0.32 ± 0.10a0.32 ± 0.08a
YBMAE1.260.56 ± 0.050.52 ± 0.07c0.49 ± 0.07c0.55 ± 0.05d
3.060.54 ± 0.050.45 ± 0.07a0.53 ± 0.07d0.55 ± 0.04d
6.060.64 ± 0.060.48 ± 0.08a0.49 ± 0.08ac0.59 ± 0.06d
PNS0.060.50 ± 0.070.45 ± 0.060.49 ± 0.06c0.52 ± 0.07c
Effect of YBMAE on microcirculation status, blood color and vessel wall clarity of rat mesentery

The microcirculation condition of the control group changed from linear to linear granular flow after amniotic fluid injection. At 30 min, 90% of the vessels turned to linear granular flow, the blood color was dark red, the vessel wall was not clear, and there was stasis in the venous blood. At 12 h, 50% of the animals died. In the three YBMAE groups and PNS group, 40%, 10%, 0% and 0% vessels had linear granular flow after amniotic fluid injection. The blood color changed from bright red to dark red in about 30%, 20%, 20%, 10% vessels respectively. Thirty minutes later, it returned to normal, and the vessel walls became clear. No animals died within 12 h.

DISCUSSION

Recent studies showed that the flow velocity, blood flow, flow status, the agglutination ability of platelet and red blood cell, the amount of opening capillary were important factors determining the functional status of the flow velocity, improve the blood flow status, the blood color, the vessel wall clarity, increased the amount of opening capillaries[6]. The results of this study demonstrated that YBMAE could increase the blood flow, and could improve the microcirculation status. The increment of flow velocity and blood flow were proportional to the amount of YBMAE before and 30 min after amniotic fluid injection. According to the literature, taurine could regulate Ca2+ metabolism and prevent arterial atherosclerosis[7]. EPA could inhibit the blood vessel constriction induced by norepinephrine, and vasoconstrictin A2 and increase elasticity of RBC, decrease the blood viscosity and synthesis of TXA2[8]. YBMAE contains plenty of taurine, EPA, amino acid and unsaturated lipid acid, therefore the effect of YBMAE in improving microcirculation may be related to its components.

Footnotes

One of the National “8.5” Key Projects of Scientific and Technical Researches, No.85-08-07-09.

References
1.  Health Department of Logistic Ministry of Navy, PLA, China. Pharmaco-logical marine creature. 1st ed. Shanghai:. Shanghai People's Publication. 1977;68-69.  [PubMed]  [DOI]
2.  Ming L, Shao BQ, Zhang Y, Li WP, Xu SY. The influence of YBMAE on quail experimental atherosclerosis. Chin Pharmacol Bull. 1996;12:554-556.  [PubMed]  [DOI]
3.  Xu SY (editor). Pharmacological experimental methodology. 2nd ed. Beijing People's Health Publishing House. 1994;992-1003.  [PubMed]  [DOI]
4.  Li YK (editor). Pharmacological experimental methodology. 1st ed. Shanghai: Shanghai Science and Technology Publishing House 1991; 141-147.  [PubMed]  [DOI]
5.  Xu SK (editor) Antiaging material media. 1st ed. Beijing: China Medical and Pharmacentical Science and Technology Press 1994; 185-193.  [PubMed]  [DOI]
6.  Pang DW, Liu MY, Tan JM, Li ZY, Shi SH. The influence of aspirin and re-ephedrine on the microcirculation of white mouse mesentery. Chin Pharmacol Bull. 1996;12:356.  [PubMed]  [DOI]
7.  Wang SN, Zhang HL, Wu DC, Wang H. The pharmacological research ad-vancement and clinical usage of taurine. China Pharmacy. 1993;4:33-34.  [PubMed]  [DOI]
8.  Wu BJ (editor) Pharmacology of animal biochemical medicine. 1st ed. Beijing: China Commercial Press 1993; 54-63.  [PubMed]  [DOI]