Endotoxins enhance hepatocarcinogenesis induced by oral intake of thioacetamide in rats

Table 3 DNA ploidy and incidence of hepatoma in various groups

Groups	n	DNA index	Rate of DNA aneuploid%	Incidence of hepatoma
				Visible		Under microscopy
				(%)	n	(%)	n
Sham	5	1.0	0	0	(0/5)	0	(0/5)
TAA control	6	1.15 ± 0.21	50	17	(1/6)	50	(3/6)
TAA + LPS	6	1.24 ± 0.25a	50	33	(2/6)	50	(3/6)
TAA + ST	6	1.44 ± 0.15ab	100	50	(3/6)	67	(4/6)
TAA + ST + LPS	6	1.56 ± 0.07abc	100	67	(4/6)	67	(4/6)

^aP < 0.02, vs TAA control group;

^bP < 0.02, vs TAA + LPS group;

^cP < 0.02, vs TAA + ST group. DNA index = mean channels of G0/1 peak in the treated rat ÷ mean channels of G0/1 peaks in the normal controls. 10⁴ cells of a rat liver were examined by flow cytometry. Rats were treated as described in Materials and Methods.