Brief Report
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 1997; 3(4): 237-237
Published online Dec 15, 1997. doi: 10.3748/wjg.v3.i4.237
Effect of garlic on micronuclei frequency in peripheral blood lymphocytes of rats with N-methyl-N’nitro-N-nitrosoguanidine-induced gastric carcinoma and precancerous lesions
Qi Su, Zhao-Yang Luo, Yang-Gui Ou, Yi-Qin Li, Jian-Guo Zhou, Dan Zhang
Qi Su, Zhao-Yang Luo, Yang-Gui Ou, Yi-Qin Li, Jian-Guo Zhou, Dan Zhang, Institute of Oncology, Hengyang Medical College, Hengyang 421001, Hunan Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Qi Su, Institute of Oncology, Hengyang Medical College, Hengyang 421001, Hunan Province, China
Received: March 13, 1997
Revised: April 22, 1997
Accepted: June 18, 1997
Published online: December 15, 1997

Abstract

AIM: To investigate the effects of garlic on micronuclei frequency (MNF) in peripheral blood lymphocytes (PBLs) of Wistar rats with N-methyl-N’nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinoma (GC) and precancerous lesions (PLs).

METHODS: Wistar rats were exposed to MNNG at 1.25 mg/5 mL per day for 10 mo to induce GC and PLs (MNNG group, n = 30); rats not exposed to MNNG served as unmodeled controls (control group, n = 16). The MNNG rats were randomly divided into a prevention treatment group (n = 30; receiving 10 mL of a 10% garlic solution per day) and an untreated model control group (n = 20; receiving tap water). MNF in PBLs were detected at experiment months 10 and 16 mo by the microculture technique.

RESULTS: The MNNG group had similar MNF levels at months 10 and 16. Compared to the control group, the MNNG, prevention and untreated model control groups had remarkably higher MNF levels (P < 0.01). The level of PLs was significantly lower in the prevention treatment group than in the untreated model control group (P < 0.01). The prevention group showed significantly lower MNF than the MNNG group (P < 0.01), and the MNF level was reduced in month 16 compared to month 10 (P < 0.01). However, the difference in MNF levels in groups given prevention or treatment was not significant.

CONCLUSION: MNNG exposure exerted continuous mutagenicity and carcinogenicity properties on PBLs, and garlic exerted a remarkable anti-mutagenic and anti-carcinogenic effect. MNF in PBL may be a novel marker of early-stage GC.

Key Words: Garlic/Pharmacology, Stomach neoplasms/zhongyi-yaoliaofa, Adenocarcinoma/Zhongyiyaoliaofa, Precancerous conditions/zhongyiyaoliaofa, Lymphocytes/Drug effects, Micronuclei/Drug effects



Footnotes

Original title: China National Journal of New Gastroenterology (1995-1997) renamed World Journal of Gastroenterology (1998-).

S- Editor: A L- Editor: Filipodia E- Editor: Li RF

$[References]