P- Reviewer: Abenavoli L, Green JT, Guandalini S, Naser SA S- Editor: Ma YJ L- Editor: A E- Editor: Zhang DN
Published online Sep 28, 2014. doi: 10.3748/wjg.v20.i36.12809
Revised: March 12, 2014
Accepted: May 23, 2014
Published online: September 28, 2014
Helicobacter pylori (H. pylori) is a gram-negative pathogen that is widespread all over the world, infecting more than 50% of the world’s population. It is etiologically associated with non-atrophic and atrophic gastritis, peptic ulcer and shows a deep association with primary gastric B-cell lymphoma and gastric adenocarcinoma. Recently, the medical research focused on the modification of the gastric environment induced by H. pylori infection, possibly affecting the absorption of nutrients and drugs as well as the production of hormones strongly implicated in the regulation of appetite and growth. Interestingly, the absorption of iron and vitamin B12 is impaired by H. pylori infection, while infected subjects have lower basal and fasting serum levels of ghrelin and higher concentration of leptin compared to controls. Since leptin is an anorexigenic hormone, and ghrelin stimulates powerfully the release of growth hormone in humans, H. pylori infection may finally induce growth retardation if acquired very early in the childhood and in malnourished children. This review is focused on the nutritional effects of H. pylori infection, such as the reduced bioavailability or the malabsorbption of essential nutrients, and of gastrointestinal hormones, as well as on the relationship between H. pylori and the metabolic syndrome.
Core tip: This review analyzes in a very comprehensive way all aspects related to nutrition and metabolism induced by Helicobacter pylori (H. pylori). Interestingly, this bacterium is able to produce different biological effects on hormones controlling both appetite and growth, mostly depending on the time of acquisition of the infection and of eradication. On the other hand, H. pylori is able to induce malabsorption of several nutrients, with a strong effect on nutrition.
Citation: Franceschi F, Tortora A, Di Rienzo T, D’Angelo G, Ianiro G, Scaldaferri F, Gerardi V, Tesori V, Lopetuso LR, Gasbarrini A. Role of
Helicobacter pyloriinfection on nutrition and metabolism. World J Gastroenterol 2014; 20(36): 12809-12817
- URL: https://www.wjgnet.com/1007-9327/full/v20/i36/12809.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i36.12809
Helicobacter pylori (H. pylori) is a gram-negative pathogen that is widespread all over the world, infecting more than 50% of the world’s population, with a predominant distribution in developing countries (up to 80%) compared to industrialized ones (20%-80%). The essential way of transmission is the inter-human contact. Poor socio-economic condition is an important risk factor. Although the infection is largely diffused, only 10%-20% of patients develop clinical manifestations[1-3].
H. pylori colonizes the entire gastric epithelium, and has an important urease activity, that leads to the ammonia production in order to protect itself from gastric acidity. It produces also other enzymes, such as phospholipase A2 and C, and glycosulfatase, which play a role in the development of the gastric mucosal damage. Indeed, H. pylori induces an inflammatory response through the gastric epithelium, with production of pro-inflammatory cytokines, such as interleukin 1β and interleukin 8. Some H. pylori genotypes, especially those vacuolating toxin A (Vac-A) and cytotoxin-associated gene A (Cag-A) positive, are associated with greater pathogenicity and more severe disease. Cag-A positive strains induce a stronger inflammatory response of gastric mucosa, with increased production of pro-inflammatory cytokines. The VacA gene, which leads to vacuolization and apoptosis of gastric epithelial cells, is genetically expressed in every H. pylori strain, even if is phenotypically present in only 60% of them. H. pylori is etiologically associated with non-atrophic and atrophic gastritis and peptic ulcer (especially duodenal ulcer). Moreover, there is a deep association between H. pylori and primary gastric B-cell lymphoma (mucosa-associated-lymphatic-tissue or MALT-lymphoma) and gastric adenocarcinoma. H. pylori has been therefore classified by IARC/WHO as “group 1 carcinogen”. Finally, over the last years, many authors investigated the relationship between H. pylori infection and extra-digestive diseases, following the rationale that it may act as an immunological trigger. The strongest association has been found with idiopathic thrombocytopenic purpura, while other studies seems to connect H. pylori infection with autoimmune diseases (Schonlein-Henoch purpura, Sjogren syndrome, autoimmune thrombocytopenia), skin diseases (urticaria, rosacea, and alopecia areata), and cardiovascular diseases (chronic ischemic heart disease and chronic ischemic cerebrovascular disease)[6,7]. Recently, the medical research focused on the modification of gastric environment induced by H. pylori infection. For example, it can affect the absorption of nutrients and drugs. In fact, idiopathic sideropenic anemia is strongly related to H. pylori infection[8,9]. The aim of this review is to evaluate the nutritional effects of H. pylori infection, such as the reduced bioavailability or the malabsorption of essential nutrients, and of gastrointestinal (GI) hormones, and, moreover, the relationship between H. pylori and metabolic imbalance conditions, such as the metabolic syndrome.
Several epidemiological studies have revealed an association between H. pylori infection and iron deficiency anemia[8,9]. According to a recent meta-analysis, iron deficiency anemia is significantly more prevalent in paediatric subjects with H. pylori than negative controls, although the correlation is less marked in adult. H. pylori-related gastritis and its effects on gastric physiology, affecting the normal process of iron absorption, may possibly explain this phenomenon; however, H. pylori is probably responsible of iron deficiency anemia through several mechanisms. Hypochlorhydria might induce the conversion of ascorbic acid to dehydroascorbic acid - a less active form hampering the promotion of iron absorption; moreover, the reduction of the ferric to ferrous form, which is fundamental for the absorption of non-heme iron, might be impaired by H. pylori infection, that cause an increase of gastric pH through the atrophy of gastric glands and fundic mucosa and the consequent decreases in gastric acid secretion[12,13]. Since iron is an essential growth factor for H. pylori, it also competes with the host for iron absorption: H. pylori possesses some proteins of the outer membrane that play a role in bacterial iron absorption as well as intracellular storage proteins with similar characteristics as ferritin. The association between iron deficiency anemia and H. pylori infection is so strong that a test and treat strategy for H. pylori infection is strongly recommended by Maastricht III European guidelines in patients with unexplained sideropenic anemia.
H. pylori infection can cause a deficiency of vitamins (such as vitamin C, vitamin A, α-tocopherol, vitamin B12 and folic acid) and essential minerals.
Vitamin B12:H. pylori infection might impair the absorption of vitamin B12 from food, leading to pernicious anemia[16,17]. Dietary cobalamin is bound to other proteins, and its release is closely related to the gastric pH status. Food-cobalamin malabsorption is characterized by the inability to absorb food-bound or protein-bound cobalamin by patients normally capable of absorbing free cobalamin. Probably, antacid drugs used by infected symptomatic subjects and the modification of the intragastric pH caused by H. pylori are the principal factors of malabsorption of vitamin B12. Annibale et al described the presence of H. pylori-related gastritis as the unique pathological finding in 57.1% of patients with macrocytic anemia caused by B12 deficiency; the majority (76%) of the patients reported a classic pernicious anemia due to atrophy of the gastric body, with associated hypergastrinemia and hypo-achlorhydria. H. pylori may also act as a molecular mimicker, as antibodies directed against the H+, K+-adenosine triphosphate protein may be evoked by a similar antigen expressed by H. pylori. Hyperhomocysteinemia related to vitamin B12 deficiency may constitute a risk for ischemic heart disease and cerebrovascular diseases. This phenomenon would therefore be the link between H. pylori infection and vascular diseases.
β-carotene:β-carotene it is the most abundant form of pro-vitamin A, being widely present in fruit and vegetables. It is able, together with its metabolites, to neutralize reactive oxygen compounds produced by oxidative stress. The bioavailability of β-carotenes depend on several factors such as the processing and cooking of foods, the composition of the nutritional matrix, and the GI health status. Hypo/achlorhydria significantly decreases bioavailability of β-carotene. β-carotene gastric mucosal concentration has been found to be markedly decreased in patients with gastric atrophy and intestinal metaplasia. In H. pylori positive subjects, the concentration of β-carotene in gastric juice is decreased, even if plasma levels are similar to controls. Probably, H. pylori reduces β-carotene bioavailability as a consequence of the slow movement of the micelle containing the vitamin through the membrane of the enterocytes due to its extreme negative charge derived from a non-acid medium.
Vitamin C: Vitamin C is actively absorbed from the small intestine via SVCTs and GLUTs transporters. Ascorbic acid is a water-soluble antioxidant that neutralizes nitrite-derived mutagens, with consequent protection against carcinogenesis. In a large study (more than 1100 patients), vitamin C plasma concentration was 20% lower in H. pylori infected subjects than in negative controls, even after correction for confounding factors, such as smoking and dietary habits. Probably, H. pylori infection may cause an irreversible inactivation of the ingested vitamin C in the intestinal lumen prior to its absorption. When hypochlorydria occurs, such as in the case of gastric atrophy, intragastric pH levels increase, and ascorbic acid is converted to the less active form of dehydroascorbic acid. Intragastric pH is then the key factor for the observed depletion of gastric juice vitamin C levels, in patients with gastric atrophy.
Vitamin E: Vitamin E includes tocopherols and tocotrienols, two classes of compounds with different biological activities. α-tocopherol is the most common alimentary form of vitamin E, representing the most important liposoluble antioxidant of the biological membranes. It is able to increase natural killer cell activity, playing an important immunologic role. In patients suffering from H. pylori infection, the mucosal concentration of α-tocopherol of the corpus is lower than that of the antrum or duodenum; probably, this phenomenon reflects a mobilization of antioxidant defenses to the sites of greatest gastric inflammation. Moreover, the presence of intestinal metaplasia and gastric atrophy is significantly associated with reduced mucosal concentration of α-tocopherol. Furthermore, mucosal concentrations of α-tocopherol in the gastric antrum decreases progressively when antral mucosal histology changes from normal to chronic gastritis alone and finally to atrophy and intestinal metaplasia.
Folate: Few studies have investigated the relationship between folates and H. pylori infection. Some authors reported a negative relationship between H. pylori infection and folate metabolism in adults. A decrease in folate absorption may take place as a consequence of decreased concentration of vitamin C in gastric juice and/or an increased level of intragastric pH, as frequently occurs in H. pylori infection.
Selenium: Selenium is a co-factor of glutathione peroxidase, which protects membranes from oxidative damage. Selenium deficiency exposes most tissues to oxidative damage. Plasma selenium levels have been showed to be similar in patients with H. pylori-related gastritis and healthy controls, even if in the first group selenium levels in the gastric tissue were significantly higher, probably because of the presence of elevated reactive oxygen species (ROS) associated with the infection. A similar behavior in gastric selenium levels may also occur as a reaction to any damage that leads to increase of ROS in the gastric mucosa. On the other side, selenium concentration is markedly decreased in the antral mucosa of patients with atrophic gastritis.
Ghrelin, a 28-amino-acid peptide produced in the gastric oxyntic gland of the fundic mucosa, is an endogenous ligand of the growth hormone (GH) secretagogue receptor, and stimulates powerfully the release of GH in humans. Other properties are the increase of appetite, facilitation of fat storage, modulation of energy homeostasis[33-35]. The effect of ghrelin on appetite and food intake is believed to be primarily mediated by peripheral input at the arcuate nucleus and further spread to the nucleus tracti solitari. Ghrelin is involved in the hypothalamic regulation of metabolic control and energy balance. Since the structure of ghrelin resembles motilin, its secretion may be associated with gastric motility and acid secretion in addition to appetite regulation[36-38]. Leptin, is an anorexigenic hormone, with opposite effect of ghrelin, inhibiting both gastric ghrelin secretion and ghrelin-dependent feeding stimulation. Secretion of ghrelin is inhibited also by insulin, growth hormone, insulin-like growth factor-I, and a high-fat diet, while both a low protein diet and fasting result in an increased secretion of ghrelin[37,38,40]. Also H. pylori infection has been hypothesized to play a role in ghrelin levels’ modulation. A recent systematic review evaluated the studies investigating this relationship. Interestingly, circulating ghrelin levels were lower in H. pylori positive patients compared to negative controls in the majority of the trials analyzed.
The effect of H. pylori on ghrelin production has been related also to H. pylori virulence. Patients with type I strain H. pylori, expressing CagA and VacA, have lower circulating ghrelin levels than those with the less virulent type II strain. Probably, the extent of gastric damage and the duration of the infection play a key role in modulation of ghrelin levels by H. pylori. Similar data have been reported also in patients with autoimmune gastritis. However, we have to consider that ghrelin production may also be replaced by other sources.
The effect of H. pylori infection has been studied also in relationship with the production of other gastric hormones. Serum gastrin concentrations were significantly increased in both H. pylori positive children and adults. Dyspeptic symptoms, including upper GI symptoms accompanied by lost or reduced appetite, were reported by the majority of H. pylori-positive children (65%), but only by 15% of H. pylori negative children. Subjects with H. pylori infection have lower basal and fasting serum levels of ghrelin and higher concentration of leptin than controls. Briefly, the gastric mucosal damage due to H. pylori provokes a marked increase of leptin and gastrin levels, and a reduction in ghrelin plasma levels, and this phenomenon may contribute to the occurrence of dyspeptic symptoms and appetite alteration.
Many studies investigated on the possible association between H. pylori infection and metabolic syndrome and atherosclerotic cardiovascular disease.
Insulin-resistance (IR) and subsequent hyperinsulinemia are the main pathogenic factors in metabolic syndrome. IR is characterized by a complex clinic scenario which includes type 2 diabetes mellitus, central obesity, dyslipidemia, hypertension, non-alcoholic fatty liver disease (NAFLD), hypertension and endothelial dysfunction. Two parameters can be used to quantify IR: body mass index (BMI) calculated as weight (kg)/[height (m)]2, and HOMA-IR (homeostasis model assessment estimates steady state beta cell function and insulin sensitivity calculated as [fasting insulin (mU/L) × fasting glucose (mmol/L)]/22.5). There is no currently widely accepted normal range for HOMA-IR, but the upper cut-off value has been proposed to be between 2.0 and 3.0. Many studies showed that H. pylori infection is linked with a higher serum oxidative stress and lower serum total antioxidant capacity and could be an independent predictor for HOMA-IR. Contrasting results emerge by studies on the effect of eradication therapy on HOMA-IR[50,51]. A case report of an 84-year-old Japanese man with IR and immune thrombocytopenic purpura showed improvement of both conditions after H. pylori eradication, with no longer need of diabetes treatment. The link between H. pylori and IR is not already clarified, but many pathogenic mechanisms have been suggested: (1) pro-inflammatory and vasoactive substances, such as cytokines [tumor necrosis factor (TNF)-α, interferon-γ, interleukin (IL)-1, IL-6, IL-8, IL-10, IL-12], eicosanoids (leukotrienes, prostaglandins), and acute phase proteins (fibrinogen, C-reactive protein) are released in infection and involved in the pathogenesis of IR; (2) enhanced platelet activation and platelet-leukocyte aggregation; (3) alteration of apoptotic process; (4) oxidative stress. H. pylori infection causes inflammation, accumulation of ROS, and oxidative DNA damage. Enhanced ROS levels due to neutrophil infiltration and increased oxidative DNA damage have been reported in gastric mucosa of H. pylori-infected patients; (5) reduction of vitamin B12 and folate concentrations, due to the chronic atrophic gastritis, and the consequent increase of homocysteine; and (6) H. pylori infection has been associated with lower ghrelin and increased leptin levels, which are associated with impaired energy homeostasis, lipid metabolism, elevated fasting insulin levels and insulin sensitivity.
Moreover, fetuin A, another acute-phase glycoprotein, can be associated to H. pylori infection and insulin resistance. This molecule is involved in mineralization and insulin signaling regulation. Its dysregulation results in an excessive inhibition of insulin signaling in the liver and skeletal muscle. Kebapcilar et al reported lower baseline serum fetuin-A levels in H. pylori-positive patients vs negative. Furthermore, fetuin-A levels appear significantly increased after successful H. pylori eradication treatment.
Several studies have demonstrated that H. pylori might be implicated in the change in serum lipid concentration, increasing the risk of atherosclerosis[61-64], but these data are not confirmed by other studies[65,66].
Kim et al conducted a study on 454 elderly Koreans, showing that H. pylori was independently associated with increase of low-density lipoprotein (LDL) cholesterol, with a correlation between LDL levels and infection severity.
Satoh et al, instead, showed a difference based on gender: studying 6289 Japanese subjects, it has been revealed that LDL and high-density lipoprotein (HDL) cholesterol were significantly higher and lower in H. pylori seropositive male subjects, while this association was not significant in female subjects. C-reactive protein values did not differ between H. pylori-positive and H. pylori-negative subjects. Another study tried to investigate circulating and gastric mucosal levels of IL1-alpha, IL-6, IL-8 and TNF-α in patients with ischemic heart disease (IHD) and matched controls, according to the presence or absence of active H. pylori infection. The results of the present study provide evidence that active H. pylori infection may play a role as a trigger factor in the pathophysiology of IHD by inducing an inflammatory cascade concentrated on gastric mucosa.
In an Italian study, infected subjects showed increased levels of cholesterol, LDL-cholesterol, and cholesterol/HDL-cholesterol atherogenic index and significant difference between infected and uninfected subjects was found in lipoprotein(a) levels.
The relationship between obesity and H. pylori infection is controversial. Obesity can alter innate and adaptive immunity, with relation between grade of obesity and immunity deterioration. Morbidly obese subjects have lower maturation of monocytes into macrophages and reduced polymorphonuclear bactericidal capacity. Severely obese patients have a signiﬁcant decrease in NK cells activity in comparison to normal individuals matched for age and gender.
According to some studies, the risk of H. pylori infection does not increase in overweight young persons and H. pylori positivity or CagA antibody status are not associated with the BMI or fasting serum leptin levels[71-73].
However, there are data demonstrating that the eradication of H. pylori significantly increases the incidence of obesity in patients with peptic ulcer disease, since it increases the level of BMI, and/or enhances the appetite of asymptomatic patients, due to an elevation of plasma ghrelin and a reduction of leptin levels[38,74-77].
A potential relationship between insulin-resistance, NAFLD and H. pylori appears to exist based on the following points: (1) NAFLD is the hepatic component of metabolic syndrome and insulin-resistance regarded as its key pathogenic hallmark; (2) patients with NAFLD present a significant increase in gut permeability, and this data is positively related with liver fat accumulation; and (3) H. pylori infection has been implicated in the pathogenesis of IR by many mechanisms, in particular: increased level of pro-inflammatory cytokines, eicosanoids, acute phase proteins, reactive oxygen species production, and cytokine serum changes (i.e., low adiponectin, ghrelin and leptin levels; high TNF-α).
In conclusion in H. pylori negative patients, obesity and metabolic syndrome mostly depend on genetic and lifestyle habits. If H. pylori is acquired very early in the childhood (as in developing countries) it may lead to malnutrition and growth retardation especially when either food intake or variety is poor.
According to some authors H. pylori can play a role on the balance of nutritional status. The incidence of H. pylori infection in childhood in developing countries is high[79-81] and in some studies H. pylori has been correlated with malnutrition and growth retardation[82,83]. Contracting H. pylori infection in childhood may result in a series of events that influence morbidity and mortality. H. pylori is associated with hypochlorhydria both in adults and in children[85-87] hypochlorhydria impairs the absorption of several nutrients and increases susceptibility to enteric infections such as giardiasis, cholera, typhoid and non-typhoidal salmonellosis[88-91] and other microorganisms, particularly in areas where they are endemic. The resultant diarrhea[85-91] may lead to malnutrition and growth retardation in children[92-94]. In conclusion, H. pylori could be associated with childhood malnutrition in developing countries both because of increased susceptibility to enteric infections caused by hypochlorhydria and because of malabsorption of nutrients.
In a recent metanalysis of randomized controlled trials comparing H. pylori eradication therapy plus oral iron supplementation to oral iron supplementation alone in subjects with H. pylori infection and iron deficiency anemia, the combination therapy showed a statistically significant increase of serum iron, serum ferritin and hemoglobin, than iron supplementation alone; those results were strongest in patients with hemoglobin levels lower than 9 g/dL. Moreover, eradication of H. pylori was significantly associated with healing of iron deficiency anemia even in patients not receiving iron supplementation therapy[96,97].
Furthermore, H. pylori eradication has been showed to improve not only iron absorption but also vitamin B12 absorption, and the successful eradication of H. pylori restored the juice/plasma ascorbic acid ratio.
On the other hand, the eradication of H. pylori has not shown efficacy on the modulation of vitamin A or vitamin E levels in serum or gastric juice.
The role of H. pylori eradication on ghrelin levels has been recently evaluated by systematic review of literature, without conclusive statements. However, a recent study showed that ghrelin did not change during the first 4 wk after H. pylori eradication, but gradually increased after 6 mo of follow-up.
Finally, in a trial by Furuta et al, body mass index and fasting blood glucose levels were not significantly modified by H. pylori eradication, while serum levels of total protein and albumin and serum total cholesterol levels significantly increased only in subjects with successful eradication; according to authors, the restoring of gastric pH that follows eradication is probably responsible for this phenomenon.
Overall, H. pylori has an exceptional impact on GI system. It is able to influence all the vital pathways of human system. Increasing evidences are focusing on its role also in pathological aspects not immediately related to the GI tract, such as metabolic syndrome and gynecological diseases. This new approach in studying H. pylori has obvious therapeutic implications and could lead to the screening of H. pylori in these diseases, especially in metabolic syndrome. Finally H. pylori, the most studied bacteria in GI tract, represents a model to follow for studying the gut microbiota properties. This could open the discussion on new diagnostic and therapeutical approaches of GI and extra-intestinal diseases.
|1.||Dzierzanowska-Fangrat K, Dzierzanowska D. Helicobacter pylori: microbiology and interactions with gastrointestinal microflora. J Physiol Pharmacol. 2006;57 Suppl 3:5-14. [PubMed]|
|2.||Malfertheiner P, Chan FK, McColl KE. Peptic ulcer disease. Lancet. 2009;374:1449-1461. [PubMed] [DOI]|
|3.||Gaetano C, Aldo B, editors . Infezione gastrica da Helicobacter pylori. Trattato di Medicina Interna: Piccin Edizioni 2009; .|
|4.||Yamaoka Y. Mechanisms of disease: Helicobacter pylori virulence factors. Nat Rev Gastroenterol Hepatol. 2010;7:629-641. [PubMed] [DOI]|
|5.||Pandey R, Misra V, Misra SP, Dwivedi M, Kumar A, Tiwari BK. Helicobacter pylori and gastric cancer. Asian Pac J Cancer Prev. 2010;11:583-588. [PubMed]|
|6.||Gasbarrini A, Franceschi F, Armuzzi A, Ojetti V, Candelli M, Torre ES, De Lorenzo A, Anti M, Pretolani S, Gasbarrini G. Extradigestive manifestations of Helicobacter pylori gastric infection. Gut. 1999;45 Suppl 1:I9-I12. [PubMed] [DOI]|
|7.||Solnick JV, Franceschi F, Roccarina D, Gasbarrini A. Extragastric manifestations of Helicobacter pylori infection--other Helicobacter species. Helicobacter. 2006;11 Suppl 1:46-51. [PubMed] [DOI]|
|8.||Capurso G, Marignani M, Delle Fave G, Annibale B. Iron-deficiency anemia in premenopausal women: why not consider atrophic body gastritis and Helicobacter pylori role? Am J Gastroenterol. 1999;94:3084-3085. [PubMed] [DOI]|
|9.||Akcam M, Ozdem S, Yilmaz A, Gultekin M, Artan R. Serum ferritin, vitamin B(12), folate, and zinc levels in children infected with Helicobacter pylori. Dig Dis Sci. 2007;52:405-410. [PubMed] [DOI]|
|10.||Qu XH, Huang XL, Xiong P, Zhu CY, Huang YL, Lu LG, Sun X, Rong L, Zhong L, Sun DY. Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis. World J Gastroenterol. 2010;16:886-896. [PubMed]|
|11.||Annibale B, Capurso G, Martino G, Grossi C, Delle Fave G. Iron deficiency anaemia and Helicobacter pylori infection. Int J Antimicrob Agents. 2000;16:515-519. [PubMed] [DOI]|
|12.||Capurso G, Lahner E, Marcheggiano A, Caruana P, Carnuccio A, Bordi C, Delle Fave G, Annibale B. Involvement of the corporal mucosa and related changes in gastric acid secretion characterize patients with iron deficiency anaemia associated with Helicobacter pylori infection. Aliment Pharmacol Ther. 2001;15:1753-1761. [PubMed] [DOI]|
|13.||Annibale B, Capurso G, Lahner E, Passi S, Ricci R, Maggio F, Delle Fave G. Concomitant alterations in intragastric pH and ascorbic acid concentration in patients with Helicobacter pylori gastritis and associated iron deficiency anaemia. Gut. 2003;52:496-501. [PubMed] [DOI]|
|14.||Pérez-Pérez GI, Israel DA. Role of iron in Helicobacter pylori: its influence in outer membrane protein expression and in pathogenicity. Eur J Gastroenterol Hepatol. 2000;12:1263-1265. [PubMed] [DOI]|
|15.||Malfertheiner P, Megraud F, O’Morain C, Bazzoli F, El-Omar E, Graham D, Hunt R, Rokkas T, Vakil N, Kuipers EJ. Current concepts in the management of Helicobacter pylori infection: the Maastricht III Consensus Report. Gut. 2007;56:772-781. [PubMed] [DOI]|
|16.||Stopeck A. Links between Helicobacter pylori infection, cobalamin deficiency, and pernicious anemia. Arch Intern Med. 2000;160:1229-1230. [PubMed] [DOI]|
|17.||Kaptan K, Beyan C, Ural AU, Cetin T, Avcu F, Gülşen M, Finci R, Yalçín A. Helicobacter pylori--is it a novel causative agent in Vitamin B12 deficiency? Arch Intern Med. 2000;160:1349-1353. [PubMed] [DOI]|
|18.||Annibale B, Capurso G, Delle Fave G. Consequences of Helicobacter pylori infection on the absorption of micronutrients. Dig Liver Dis. 2002;34 Suppl 2:S72-S77. [PubMed]|
|19.||Howden CW. Vitamin B12 levels during prolonged treatment with proton pump inhibitors. J Clin Gastroenterol. 2000;30:29-33. [PubMed] [DOI]|
|20.||Cohen H, Weinstein WM, Carmel R. Heterogeneity of gastric histology and function in food cobalamin malabsorption: absence of atrophic gastritis and achlorhydria in some patients with severe malabsorption. Gut. 2000;47:638-645. [PubMed] [DOI]|
|21.||Claeys D, Faller G, Appelmelk BJ, Negrini R, Kirchner T. The gastric H+,K+-ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy. Gastroenterology. 1998;115:340-347. [PubMed] [DOI]|
|22.||Tang G, Serfaty-Lacrosniere C, Camilo ME, Russell RM. Gastric acidity influences the blood response to a beta-carotene dose in humans. Am J Clin Nutr. 1996;64:622-626. [PubMed]|
|23.||Sanderson MJ, White KL, Drake IM, Schorah CJ. Vitamin E and carotenoids in gastric biopsies: the relation to plasma concentrations in patients with and without Helicobacter pylori gastritis. Am J Clin Nutr. 1997;65:101-106. [PubMed]|
|24.||Zhang ZW, Patchett SE, Perrett D, Domizio P, Farthing MJ. Gastric alphα-tocopherol and beta-carotene concentrations in association with Helicobacter pylori infection. Eur J Gastroenterol Hepatol. 2000;12:497-503. [PubMed] [DOI]|
|25.||Ziegler EE, Filer LJ. Present Knowledge in Nutrition. 6th ed. North America: International Life Sciences Institute 1990; .|
|26.||Waring AJ, Drake IM, Schorah CJ, White KL, Lynch DA, Axon AT, Dixon MF. Ascorbic acid and total vitamin C concentrations in plasma, gastric juice, and gastrointestinal mucosa: effects of gastritis and oral supplementation. Gut. 1996;38:171-176. [PubMed] [DOI]|
|27.||Woodward M, Tunstall-Pedoe H, McColl K. Helicobacter pylori infection reduces systemic availability of dietary vitamin C. Eur J Gastroenterol Hepatol. 2001;13:233-237. [PubMed] [DOI]|
|28.||Capurso G, Ricci R, Panzuto F, Baccini F, Passi S, Di Giulio E, Delle Fave G, Annibale B. Intragastric ascorbic but not uric acid is depleted in relation with the increased pH in patients with atrophic body gastritis and H. pylori gastritis. Helicobacter. 2003;8:300-306. [PubMed] [DOI]|
|29.||Sies H, Stahl W. Vitamins E and C, beta-carotene, and other carotenoids as antioxidants. Am J Clin Nutr. 1995;62:1315S-1321S. [PubMed]|
|30.||Phull PS, Price AB, Thorniley MS, Green CJ, Jacyna MR. Vitamin E concentrations in the human stomach and duodenum--correlation with Helicobacter pylori infection. Gut. 1996;39:31-35. [PubMed] [DOI]|
|31.||Zullo A, Rinaldi V, Efrati C, Hassan C, Caroli S, Riggio O, Attili AF. Zinc, ammonia, and Helicobacter pylori infection in liver cirrhosis. Dig Liver Dis. 2000;32:836-838. [PubMed]|
|32.||Ustündağ Y, Boyacioğlu S, Haberal A, Demirhan B, Bilezikçi B. Plasma and gastric tissue selenium levels in patients with Helicobacter pylori infection. J Clin Gastroenterol. 2001;32:405-408. [PubMed] [DOI]|
|33.||Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature. 1999;402:656-660. [PubMed] [DOI]|
|34.||Murray CD, Kamm MA, Bloom SR, Emmanuel AV. Ghrelin for the gastroenterologist: history and potential. Gastroenterology. 2003;125:1492-1502. [PubMed]|
|35.||Cummings DE. Helicobacter pylori and ghrelin: Interrelated players in body-weight regulation? Am J Med. 2004;117:436-439. [PubMed] [DOI]|
|36.||Masuda Y, Tanaka T, Inomata N, Ohnuma N, Tanaka S, Itoh Z, Hosoda H, Kojima M, Kangawa K. Ghrelin stimulates gastric acid secretion and motility in rats. Biochem Biophys Res Commun. 2000;276:905-908. [PubMed] [DOI]|
|37.||Asakawa A, Inui A, Kaga T, Yuzuriha H, Nagata T, Ueno N, Makino S, Fujimiya M, Niijima A, Fujino MA. Ghrelin is an appetite-stimulatory signal from stomach with structural resemblance to motilin. Gastroenterology. 2001;120:337-345. [PubMed]|
|38.||Konturek PC, Cześnikiewicz-Guzik M, Bielanski W, Konturek SJ. Involvement of Helicobacter pylori infection in neuro-hormonal control of food intake. J Physiol Pharmacol. 2006;57 Suppl 5:67-81. [PubMed]|
|39.||Kalra SP, Ueno N, Kalra PS. Stimulation of appetite by ghrelin is regulated by leptin restraint: peripheral and central sites of action. J Nutr. 2005;135:1331-1335. [PubMed]|
|40.||Blaser MJ, Atherton JC. Helicobacter pylori persistence: biology and disease. J Clin Invest. 2004;113:321-333. [PubMed] [DOI]|
|41.||Nweneka CV, Prentice AM. Helicobacter pylori infection and circulating ghrelin levels - a systematic review. BMC Gastroenterol. 2011;11:7. [PubMed] [DOI]|
|42.||Isomoto H, Nishi Y, Ohnita K, Mizuta Y, Kohno S, Ueno H, Nakazato M. The Relationship between Plasma and Gastric Ghrelin Levels and Strain Diversity in Helicobacter pylori Virulence. Am J Gastroenterol. 2005;100:1425-1427. [PubMed] [DOI]|
|43.||Liew PL, Lee WJ, Lee YC, Chen WY. Gastric ghrelin expression associated with Helicobacter pylori infection and chronic gastritis in obese patients. Obes Surg. 2006;16:612-619. [PubMed] [DOI]|
|44.||Checchi S, Montanaro A, Pasqui L, Ciuoli C, Cevenini G, Sestini F, Fioravanti C, Pacini F. Serum ghrelin as a marker of atrophic body gastritis in patients with parietal cell antibodies. J Clin Endocrinol Metab. 2007;92:4346-4351. [PubMed] [DOI]|
|45.||Abiko Y, Suzuki H, Masaoka T, Nomura S, Kurabayashi K, Hosoda H, Kangawa K, Hibi T. Enhanced plasma ghrelin levels in Helicobacter pylori-colonized, interleukin-1-receptor type 1-homozygous knockout (IL-1R1-/-) mice. World J Gastroenterol. 2005;11:4148-4153. [PubMed]|
|46.||Talley NJ, Stanghellini V, Heading RC, Koch KL, Malagelada JR, Tytgat GN. Functional gastroduodenal disorders. Gut. 1999;45 Suppl 2:II37-II42. [PubMed]|
|47.||Polyzos SA, Kountouras J, Zavos C, Deretzi G. The association between Helicobacter pylori infection and insulin resistance: a systematic review. Helicobacter. 2011;16:79-88. [PubMed] [DOI]|
|48.||Aslan M, Horoz M, Nazligul Y, Bolukbas C, Bolukbas FF, Selek S, Celik H, Erel O. Insulin resistance in H pylori infection and its association with oxidative stress. World J Gastroenterol. 2006;12:6865-6868. [PubMed]|
|49.||Gunji T, Matsuhashi N, Sato H, Fujibayashi K, Okumura M, Sasabe N, Urabe A. Helicobacter pylori infection significantly increases insulin resistance in the asymptomatic Japanese population. Helicobacter. 2009;14:144-150. [PubMed] [DOI]|
|50.||Park SH, Jeon WK, Kim SH, Kim HJ, Park DI, Cho YK, Sung IK, Sohn CI, Kim BI, Keum DK. Helicobacter pylori eradication has no effect on metabolic and inflammatory parameters. J Natl Med Assoc. 2005;97:508-513. [PubMed]|
|51.||Gen R, Demir M, Ataseven H. Effect of Helicobacter pylori eradication on insulin resistance, serum lipids and low-grade inflammation. South Med J. 2010;103:190-196. [PubMed] [DOI]|
|52.||Imai J, Yamada T, Saito T, Ishigaki Y, Hinokio Y, Kotake H, Oka Y, Katagiri H. Eradication of insulin resistance. Lancet. 2009;374:264. [PubMed] [DOI]|
|53.||Yeh JJ, Tsai S, Wu DC, Wu JY, Liu TC, Chen A. P-selectin-dependent platelet aggregation and apoptosis may explain the decrease in platelet count during Helicobacter pylori infection. Blood. 2010;115:4247-4253. [PubMed] [DOI]|
|54.||Basso D, Plebani M, Kusters JG. Pathogenesis of Helicobacter pylori infection. Helicobacter. 2010;15 Suppl 1:14-20. [PubMed] [DOI]|
|55.||Evrengul H, Tanriverdi H, Kuru O, Enli Y, Yuksel D, Kilic A, Kaftan A, Kirac S, Kilic M. Elevated homocysteine levels in patients with slow coronary flow: relationship with Helicobacter pylori infection. Helicobacter. 2007;12:298-305. [PubMed] [DOI]|
|56.||Osawa H, Nakazato M, Date Y, Kita H, Ohnishi H, Ueno H, Shiiya T, Satoh K, Ishino Y, Sugano K. Impaired production of gastric ghrelin in chronic gastritis associated with Helicobacter pylori. J Clin Endocrinol Metab. 2005;90:10-16. [PubMed] [DOI]|
|57.||Roper J, Francois F, Shue PL, Mourad MS, Pei Z, Olivares de Perez AZ, Perez-Perez GI, Tseng CH, Blaser MJ. Leptin and ghrelin in relation to Helicobacter pylori status in adult males. J Clin Endocrinol Metab. 2008;93:2350-2357. [PubMed] [DOI]|
|58.||Manolakis AC, Tiaka EK, Kapsoritakis AN, Georgoulias P, Tsiopoulos F, Valotassiou V, Potamianos SP. Increased fetuin A levels in Helicobacter pylori infection: a missing link between H. pylori and insulin resistance? Diabetologia. 2011;54:472-474. [PubMed] [DOI]|
|59.||Polyzos SA, Kountouras J, Zavos C, Deretzi G. The potentially dual-faceted nature of fetuin-A in Helicobacter pylori infection and insulin resistance. Clinics (Sao Paulo). 2011;66:911-912. [PubMed] [DOI]|
|60.||Kebapcilar L, Bilgir O, Cetinkaya E, Akyol M, Bilgir F, Bozkaya G. The effect of Helicobacter pylori eradication on macrophage migration inhibitory factor, C-reactive protein and fetuin-a levels. Clinics (Sao Paulo). 2010;65:799-802. [PubMed] [DOI]|
|61.||Cullen P, Assmann G. High risk strategies for atherosclerosis. Clin Chim Acta. 1999;286:31-45. [PubMed]|
|62.||Hoffmeister A, Rothenbacher D, Bode G, Persson K, März W, Nauck MA, Brenner H, Hombach V, Koenig W. Current infection with Helicobacter pylori, but not seropositivity to Chlamydia pneumoniae or cytomegalovirus, is associated with an atherogenic, modified lipid profile. Arterioscler Thromb Vasc Biol. 2001;21:427-432. [PubMed] [DOI]|
|63.||Laurila A, Bloigu A, Näyhä S, Hassi J, Leinonen M, Saikku P. Association of Helicobacter pylori infection with elevated serum lipids. Atherosclerosis. 1999;142:207-210. [PubMed] [DOI]|
|64.||Niemelä S, Karttunen T, Korhonen T, Läärä E, Karttunen R, Ikäheimo M, Kesäniemi YA. Could Helicobacter pylori infection increase the risk of coronary heart disease by modifying serum lipid concentrations? Heart. 1996;75:573-575. [PubMed]|
|65.||Danesh J, Peto R. Risk factors for coronary heart disease and infection with Helicobacter pylori: meta-analysis of 18 studies. BMJ. 1998;316:1130-1132. [PubMed] [DOI]|
|66.||Zhu J, Quyyumi AA, Muhlestein JB, Nieto FJ, Horne BD, Zalles-Ganley A, Anderson JL, Epstein SE. Lack of association of Helicobacter pylori infection with coronary artery disease and frequency of acute myocardial infarction or death. Am J Cardiol. 2002;89:155-158. [PubMed] [DOI]|
|67.||Kim HL, Jeon HH, Park IY, Choi JM, Kang JS, Min KW. Helicobacter pylori infection is associated with elevated low density lipoprotein cholesterol levels in elderly Koreans. J Korean Med Sci. 2011;26:654-658. [PubMed] [DOI]|
|68.||Satoh H, Saijo Y, Yoshioka E, Tsutsui H. Helicobacter Pylori infection is a significant risk for modified lipid profile in Japanese male subjects. J Atheroscler Thromb. 2010;17:1041-1048. [PubMed] [DOI]|
|69.||Di Bonaventura G, Piccolomini R, Pompilio A, Zappacosta R, Piccolomini M, Neri M. Serum and mucosal cytokine profiles in patients with active Helicobacter pylori and ischemic heart disease: is there a relationship? Int J Immunopathol Pharmacol. 2007;20:163-172. [PubMed]|
|70.||Arslan E, Atilgan H, Yavaşoğlu I. The prevalence of Helicobacter pylori in obese subjects. Eur J Intern Med. 2009;20:695-697. [PubMed] [DOI]|
|71.||Kyriazanos ID, Sfiniadakis I, Gizaris V, Hountis P, Hatziveis K, Dafnopoulou A, Datsakis K. The incidence of Helicobacter pylori infection is not increased among obese young individuals in Greece. J Clin Gastroenterol. 2002;34:541-546. [PubMed] [DOI]|
|72.||Ioannou GN, Weiss NS, Kearney DJ. Is Helicobacter pylori seropositivity related to body mass index in the United States? Aliment Pharmacol Ther. 2005;21:765-772. [PubMed] [DOI]|
|73.||Cho I, Blaser MJ, François F, Mathew JP, Ye XY, Goldberg JD, Bini EJ. Helicobacter pylori and overweight status in the United States: data from the Third National Health and Nutrition Examination Survey. Am J Epidemiol. 2005;162:579-584. [PubMed] [DOI]|
|74.||Fujiwara Y, Higuchi K, Arafa UA, Uchida T, Tominaga K, Watanabe T, Arakawa T. Long-term effect of Helicobacter pylori eradication on quality of life, body mass index, and newly developed diseases in Japanese patients with peptic ulcer disease. Hepatogastroenterology. 2002;49:1298-1302. [PubMed]|
|75.||Kamada T, Hata J, Kusunoki H, Ito M, Tanaka S, Kawamura Y, Chayama K, Haruma K. Eradication of Helicobacter pylori increases the incidence of hyperlipidaemia and obesity in peptic ulcer patients. Dig Liver Dis. 2005;37:39-43. [PubMed] [DOI]|
|76.||Nwokolo CU, Freshwater DA, O’Hare P, Randeva HS. Plasma ghrelin following cure of Helicobacter pylori. Gut. 2003;52:637-640. [PubMed] [DOI]|
|77.||Loffeld RJ. Helicobacter pylori, obesity and gastro-oesophageal reflux disease. Is there a relation? A personal view. Neth J Med. 2005;63:344-347. [PubMed]|
|78.||Abenavoli L, Milic N, Masarone M, Persico M. Association between non-alcoholic fatty liver disease, insulin resistance and Helicobacter pylori. Med Hypotheses. 2013;81:913-915. [PubMed] [DOI]|
|79.||Mégraud F, Brassens-Rabbé MP, Denis F, Belbouri A, Hoa DQ. Seroepidemiology of Campylobacter pylori infection in various populations. J Clin Microbiol. 1989;27:1870-1873. [PubMed]|
|80.||Kehrt R, Becker M, Brösicke H, Krüger N, Helge H. Prevalence of Helicobacter pylori infection in Nicaraguan children with persistent diarrhea, diagnosed by the 13C-urea breath test. J Pediatr Gastroenterol Nutr. 1997;25:84-88. [PubMed] [DOI]|
|81.||Goodman KJ, Correa P, Tenganá Aux HJ, Ramírez H, DeLany JP, Guerrero Pepinosa O, López Quiñones M, Collazos Parra T. Helicobacter pylori infection in the Colombian Andes: a population-based study of transmission pathways. Am J Epidemiol. 1996;144:290-299. [PubMed] [DOI]|
|82.||Thomas JE, Dale A, Bunn JE, Harding M, Coward WA, Cole TJ, Weaver LT. Early Helicobacter pylori colonisation: the association with growth faltering in The Gambia. Arch Dis Child. 2004;89:1149-1154. [PubMed] [DOI]|
|83.||Mera RM, Correa P, Fontham EE, Reina JC, Pradilla A, Alzate A, Bravo LE. Effects of a new Helicobacter pylori infection on height and weight in Colombian children. Ann Epidemiol. 2006;16:347-351. [PubMed] [DOI]|
|84.||Windle HJ, Kelleher D, Crabtree JE. Childhood Helicobacter pylori infection and growth impairment in developing countries: a vicious cycle? Pediatrics. 2007;119:e754-e759. [PubMed] [DOI]|
|85.||Dale A, Thomas JE, Darboe MK, Coward WA, Harding M, Weaver LT. Helicobacter pylori infection, gastric acid secretion, and infant growth. J Pediatr Gastroenterol Nutr. 1998;26:393-397. [PubMed] [DOI]|
|86.||Weaver LT. Royal Society of Tropical Medicine and Hygiene Meeting at Manson House, London, 16 February 1995. Aspects of Helicobacter pylori infection in the developing and developed world. Helicobacter pylori infection, nutrition and growth of West African infants. Trans R Soc Trop Med Hyg. 1995;89:347-350. [PubMed] [DOI]|
|87.||Howden CW, Hunt RH. Relationship between gastric secretion and infection. Gut. 1987;28:96-107. [PubMed] [DOI]|
|88.||Khosla SN, Jain N, Khosla A. Gastric acid secretion in typhoid fever. Postgrad Med J. 1993;69:121-123. [PubMed] [DOI]|
|89.||Giannella RA, Broitman SA, Zamcheck N. Salmonella enteritis. I. Role of reduced gastric secretion in pathogenesis. Am J Dig Dis. 1971;16:1000-1006. [PubMed] [DOI]|
|90.||Nalin DR, Levine RJ, Levine MM, Hoover D, Bergquist E, McLaughlin J, Libonati J, Alam J, Hornick RB. Cholera, non-vibrio cholera, and stomach acid. Lancet. 1978;2:856-859. [PubMed] [DOI]|
|91.||Cook GC. Infective gastroenteritis and its relationship to reduced gastric acidity. Scand J Gastroenterol Suppl. 1985;111:17-23. [PubMed] [DOI]|
|92.||Passaro DJ, Taylor DN, Meza R, Cabrera L, Gilman RH, Parsonnet J. Acute Helicobacter pylori infection is followed by an increase in diarrheal disease among Peruvian children. Pediatrics. 2001;108:E87. [PubMed] [DOI]|
|93.||Bravo LE, Mera R, Reina JC, Pradilla A, Alzate A, Fontham E, Correa P. Impact of Helicobacter pylori infection on growth of children: a prospective cohort study. J Pediatr Gastroenterol Nutr. 2003;37:614-619. [PubMed] [DOI]|
|94.||Choe YH, Kim SK, Hong YC. Helicobacter pylori infection with iron deficiency anaemia and subnormal growth at puberty. Arch Dis Child. 2000;82:136-140. [PubMed] [DOI]|
|95.||Yuan W, Li Yumin D, Yang L. Iron deficiency anemia in Helicobacter pylori infection: meta-analysis of randomized controlled trials. Scand J Gastroenterol. 2010;45:665-676. [PubMed] [DOI]|
|96.||Barabino A, Dufour C, Marino CE, Claudiani F, De Alessandri A. Unexplained refractory iron-deficiency anemia associated with Helicobacter pylori gastric infection in children: further clinical evidence. J Pediatr Gastroenterol Nutr. 1999;28:116-119. [PubMed] [DOI]|
|97.||Kostaki M, Fessatou S, Karpathios T. Refractory iron-deficiency anaemia due to silent Helicobacter pylori gastritis in children. Eur J Pediatr. 2003;162:177-179. [PubMed] [DOI]|
|98.||Ruiz B, Rood JC, Fontham ET, Malcom GT, Hunter FM, Sobhan M, Johnson WD, Correa P. Vitamin C concentration in gastric juice before and after anti-Helicobacter pylori treatment. Am J Gastroenterol. 1994;89:533-539. [PubMed]|
|99.||Hep A, Pospísilová J, Dolina J, Prásek J, Dítĕ P. [Levels of vitamins A, E and C in serum and gastric juice in relation to gastric mucosa and occurrence of Helicobacter pylori]. Vnitr Lek. 1998;44:396-399. [PubMed]|