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World J Gastroenterol. Jul 28, 2008; 14(28): 4535-4539
Published online Jul 28, 2008. doi: 10.3748/wjg.14.4535
Celecoxib-related gastroduodenal ulcer and cardiovascular events in a randomized trial for gastric cancer prevention
Guo-Shuang Feng, Jun-Ling Ma, Benjamin CY Wong, Lian Zhang, Wei-Dong Liu, Kai-Feng Pan, Lin Shen, Xiao-Dong Zhang, Jie Li, Harry HX Xia, Ji-You Li, Shiu Kum Lam, Wei-Cheng You
Guo-Shuang Feng, Jun-Ling Ma, Lian Zhang, Kai-Feng Pan, Wei-Cheng You, Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100036, China
Benjamin CY Wong, Harry HX Xia, Shiu Kum Lam, Department of Medicine, University of Hong Kong, Hong Kong, China
Lin Shen, Xiao-Dong Zhang, Jie Li, Department of Medical Oncology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100036, China
Ji-You Li, Department of Pathology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100036, China
Wei-Dong Liu, Healthy Bureau of Linqu County, Weifang 262600, Shandong Province, China
Author contributions: Feng GS, Ma JL, Zhang L, Pan KF, and You WC contributed to the concept and designed the study on adverse events of celecoxib use; Ma JL and Liu WD collected the data; Feng GS and Ma JL wrote the manuscript; Feng GS was responsible for the statistical analysis; Shen L, Zhang XD, Li J, and Li JY contributed to the medical supervision in the trial; Xia HHX, Lam SK, and Wong BCY revised the manuscript; You WC supervised the whole study and polished the manuscript.
Correspondence to: Wei-Cheng You, MD, Department of Cancer Epidemiology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, 52 Fucheng Road, Haidian District, Beijing 100036, China. weichengyou@yahoo.com
Telephone: +86-10-88141035
Fax: +86-10-88122437
Received: April 30, 2008
Revised: June 10, 2008
Accepted: June 17, 2008
Published online: July 28, 2008

Abstract

AIM: To evaluate the long-term risk of gastroduodenal ulcer and cardiovascular events induced by celecoxib in a population-based, randomized, double-blind, placebo-controlled study.

METHODS: From 2004 to 2006, a total of 1024 Chinese patients (aged 35 to 64 years) with severe chronic atrophic gastritis, intestinal metaplasia or dysplasia were randomly assigned to receive 200 mg of celecoxib twice daily or placebo in Linqu County (Shandong Province, China), a high-risk area of gastric cancer. All gastroduodenal ulcer and cardiovascular events occurred were recorded and the patients were followed up for 1.5 years after treatment. At the end of the trial, a systematic interview survey about other adverse events was conducted.

RESULTS: Gastroduodenal ulcer was detected in 19 of 463 (3.72%) patients who received celecoxib and 17 of 473 (3.31%) patients who received placebo, respectively (odds ratio = 1.13, 95% CI = 0.58-2.19). Cardiovascular (CV) events occurred in 4 patients who received celecoxib and in 5 patients who received placebo, respectively. Compared with those who received placebo, patients who received celecoxib had no significant increase in occurrence of CV events (hazard ratio = 0.84, 95% CI = 0.23-3.15). Among the adverse events acquired by interview survey, only the frequency of bloating was significantly higher in patients treated with celecoxib than in those treated with placebo.

CONCLUSION: Treatment of gastric cancer with celecoxib is not associated with increased risk of gastroduodenal ulcer and cardiovascular events.

Key Words: Celecoxib, Gastroduodenal ulcer, Cardiovascular diseases, Adverse effects, Epidemiology, Randomized controlled trial


Citation: Feng GS, Ma JL, Wong BC, Zhang L, Liu WD, Pan KF, Shen L, Zhang XD, Li J, Xia HH, Li JY, Lam SK, You WC. Celecoxib-related gastroduodenal ulcer and cardiovascular events in a randomized trial for gastric cancer prevention. World J Gastroenterol 2008; 14(28): 4535-4539
INTRODUCTION

Celecoxib, approved by the US Food and Drug Administration (FDA) in 1998 for osteoarthritis and rheumatoid arthritis, is a cyclooxygenase-2 (COX-2) inhibitor. Owing to the selective inhibition of COX-2, this drug provides similar anti-inflammatory effects and a reduced risk of gastrointestinal complications in osteoarthritis and rheumatoid arthritis patients compared with nonsteroidal anti-inflammatory drugs (NSAIDs)[12], which inhibit both COX-1 and COX-2. In addition, celecoxib, a selective inhibitor of COX-2, can block tumor growth by its antiangiogenic and proapoptotic effects, suggesting that it can be used in the prevention and treatment of cancers[35].

However, it was reported that rofecoxib, also a COX-2 inhibitor, is associated with gastrointestinal toxic effects and cardiovascular (CV) events[67]; But, it has no gastrointestinal toxicity[8]. The conflicting results have raised the concern about the safety of celecoxib[910]. In 2005, the FDA Advisory Committee concluded that the adverse events of celecoxib are less than those of rofecoxib[11]. Therefore, we studied the safety issue of celecoxib. Gastroduodenal ulcer and CV events induced by celecoxib are reported in this paper.

MATERIALS AND METHODS
Study population

In 2004, a total of 1024 subjects, randomly selected from 12 villages of Linqu County (Shandong Province, China), participated in this study. Their age was 35-64 years. All subjects received a brief physical examination and their medical history was recorded. Subjects were ineligible if they had a history of stroke within two years, angina or congestive heart failure or myocardial infarction within one year, neoplastic diseases in the previous 10 years, esophageal or gastric surgery, inflammatory bowel disease, or bleeding diathesis, paracetamol allergy or hypersensitivity to aspirin, or other life-threatening illness. The remainders received 13C-urea breath test (13C-UBT) and gastroscopic examination with biopsies from 5 standard sites of the stomach. Only those who had Helicobacter pylori (H pylori) infection and a histological diagnosis of severe chronic atrophic gastritis (CAG), intestinal metaplasia (IM) or dysplasia (DYS) were enrolled in the intervention trial. A written informed consent was obtained from each participant and the trial was approved by the Institutional Review Board (IRB) of Peking University School of Oncology (PUSO).

Study design and randomization

Subjects were randomly assigned to received antibiotics and/or celecoxib or their placebo in a 2 × 2 factorial design. Finally, the subjects were divided into four groups. Group 1 received anti-H pylori treatment in the first week followed by 200 mg celecoxib twice daily for 24 mo, group 2 received anti-H pylori treatment in the first week followed by a look-alike celecoxib placebo for 24 mo, group 3 received a look-alike anti-H pylori placebo in the first week followed by celecoxib twice daily for 24 mo, group 4 received a look-alike anti-H pylori placebo in the first week followed by a look-alike celecoxib placebo for 24 mo. We only observed and evaluated the risk of cardiovascular and other adverse events in the celecoxib and placebo groups (Figure 1). Both the participants and investigators were blinded to the treatment. Randomization of treatment assignments was generated at Westat Inc. in the US after eligibility was determined.

Figure 1
Figure 1 Trial profile.

From March 16 to 30, 2004, the eligible participants were given a triple therapy with 20 mg omeprazole, 1 g amoxicillin and 500 mg clarithromycin or placebo twice daily for 7 d to eradicate their H pylori infection. Then 200 mg of celecoxib or placebo twice daily was given orally from April 8, 2004 to May 6, 2006, except for April 2005 because of the interim gastroscopic examination.

Follow-up

During the period of study, labeled pill bottles of celecoxib or placebo were distributed to participants in each village by PUSO staff and trained field staff each month. The field staff visited each participant twice a month to monitor treatment-related events and to promote pill compliance in the entire duration of the study. The staff counted and recorded the number of pills remaining in each bottle before the new pill bottles were distributed each month. If a subject was not at home during the staff visit, an evening visit was scheduled. A subject was considered compliant if the pill bottle was empty at the end of that month. If a subject was unable to be contacted at the time of counting pills, he or she was considered non-compliant.

Adverse events

Gastroduodenal ulcer was detected in 2005 and 2006 by the same group of PUSO physicians and gastroenterologists. Gastroscopic procedures, including biopsy samples taken from seven standard sites of stomach and histopathologic criteria, have been described elsewhere[12]. The gastroenterologist and pathologist were blinded to the subjects’ intervention.

The CV events were defined as fatal or nonfatal myocardial infarction, ischemic and hemorrhagic stroke as previously described[13]. When visiting the participants, investigators recorded the CV events and other complaints of the participants. While investigators were absent, participants-reported symptoms were recorded by doctors in village clinics. All the CV events were diagnosed in local hospitals.

Other non-adjudicated adverse events were acquired by an interview among all the subjects at the end of the trial in May 2006. All the subjects’ symptoms in the past two years were inquired and recorded by the trained interviewers, checked and categorized by two physicians in a blinded fashion after completion of the survey.

If the symptoms were related to treatment, PUSO physicians and field staff paid a close attention to the subjects for at least 2 mo and these subjects received continuous treatment if the symptoms were aggravated.

Statistical analysis

This study was designed to achieve a significant level of 95% (< 0.05) and a power of 90% to detect a 20% regression of pre-malignant lesions, based on the background of 80% prevalence of gastric atrophy. At least 120 subjects were required in each group in order to detect a significant difference between the different treatment groups.

All data analyses were performed in a blinded fashion. The relative risks (with 95% confidence intervals) of gastroduodenal ulcer were analyzed using logistic regression by adjusting gender, age, smoking and drinking. The rate of CV events was determined and multivariate hazard ratio (HR) was calculated using the Cox proportional-hazard model. All P values were two-sided, and P < 0.05 was considered statistically significant. All analyses were performed with SAS software, version 8.2.

RESULTS

The 1024 participants were divided into celecoxib treatment group (n = 511) and placebo treatment group (n = 513). The baseline characteristics were balanced between the two groups (Table 1). During the two-year period of treatment, 88 participants who were relatively evenly distributed between the two groups withdrew from the study (Figure 1). The compliance rate was 90.61% in the celecoxib treatment group and 92.20% in the placebo treatment group, respectively.

Table 1 Baseline characteristics of 1024 participants.
Celecoxib, n (%)Placebo, n (%)P
Male238 (46.58)235 (45.81)0.81
Age (yr, means ± SD)52.94 ± 6.5152.93 ± 6.480.97
Smoking146 (28.57)142 (27.68)0.75
Drinking172 (33.66)175 (34.11)0.88
Hypertension155 (30.33)160 (31.19)0.77

From April 2004 to May 2006, gastroduodenal ulcer was detected in 19 of 463 (3.72%) participants of the celecoxib treatment group and in 17 of 473 (3.31%) participants of the placebo treatment group, respectively. The odds ratio (OR) was 1.13 (95% CI = 0.58-2.19, Table 2).

Table 2 Incidence and risk of side effects in two groups.
Celecoxib, n (%)Placebo, n (%)OR (95% CI)
Gastroduodenal ulcer19 (3.72)17 (3.31)1.13 (0.58-2.22)
CV events4 (0.86)5 (1.06)0.84 (0.23-3.15)
Main nonadjudicated side effects
Abdominal pain8 (1.73)13 (2.75)0.62 (0.26-1.52)
Bloating19 (4.10)7 (1.48)2.85 (1.19-6.84)
Constipation9 (1.94)15 (3.17)0.61 (0.26-1.40)
Diarrhea24 (5.18)24 (5.07)1.02 (0.57-1.83)
Dizziness25 (5.40)36 (7.61)0.69 (0.41-1.17)
Gastric spasmus15 (3.24)15 (3.17)1.02 (0.49-2.12)
Headache26 (5.62)23 (4.86)1.16 (0.65-2.07)
Heartburn29 (6.26)23 (4.86)1.31 (0.75-2.30)
Loss of appetite25 (5.40)16 (3.38)1.63 (0.86-3.10)
Muscle pain55 (11.88)70 (14.80)0.78 (0.53-1.13)
Nausea14 (3.02)17 (3.59)0.84 (0.41-1.72)
Pain in the chest16 (3.46)17 (3.59)0.96 (0.48-1.92)
Palpitations22 (4.75)16 (3.38)1.43 (0.74-2.75)

During the entire period of follow-up, CV events occurred in 4 participants of the celecoxib treatment group and in 5 participants of the placebo treatment group, respectively (Table 2). Compared with the placebo treatment group, the celecoxib treatment group had no significant increase in occurrence of CV events (HR = 0.84, 95% CI = 0.23-3.15).

The main nonadjudicated side effects are listed in Table 2. Except for bloating (OR = 2.85, 95% CI = 1.19-6.84), there were no significant differences in the frequency of other nonadjudicated adverse events between the two groups.

DISCUSSION

In this study, gastroduodenal ulcer and CV events occurred in the subjects who took 200 mg celecoxib twice daily.

Two previous trials addressed the possibility that celecoxib has a lower rate of gastrointestinal complications than NSAIDs[1415]. It was reported that the annual incidence rate of upper gastrointestinal complications and symptomatic ulcers is significantly lower in the celecoxib treatment group than in the combined diclofenac and ibuprofen treatment group (2.08% vs 3.54%; P = 0.02) after 6 mo of treatment[14]. It has been shown that the incidence rate of gastric ulcer in the celecoxib treatment group and diclofenac treatment group is 18% and 34%, respectively (P < 0.001), and the incidence rate of duodenal ulcer is 5% and 11%, in the celecoxib treatment group and diclofenac treatment group, respectively (P < 0.009)[15].

Although the distinct role of celecoxib in ulcer is still unclear[16], most studies suggested that celecoxib is not associated with gastric or duodenal ulcer[1719]. Our trial compared the effects of celecoxib and placebo on gastroduodenal ulcer, and the risk of gastroduodenal ulcer was not increased after treatment with 200 mg celecoxib daily compared with placebo.

The association between celecoxib and CV events was still debatable in our study. It was reported that a single dose of 400 mg celecoxib daily and placebo does not induce excess CV risk[20]. However, it was reported that 800 mg celecoxib increases the risk of death due to cardiovascular disease, myocardial infarction, stroke, or heart failure[21].

The mechanism underlying the potential cardiovascular risk of COX-2 inhibitors is not fully understood. Although the imbalance caused by COX-2 inhibitors suppressing the COX-2 dependent prostacyclin production in endothelial cells without affecting the synthesis of platelet-derived thromboxane A2, may promote thrombosis and increase the risk of CV events[2224], the extent of instability to serum thromboxane and platelet function can be influenced by many factors, such as different doses of COX-2 inhibitors, variability among patients[16].

In this study, different dose-effects of celecoxib on cardiovascular risk were observed. The dose of 800 mg celecoxib daily could increase the CV risk. However, 400 mg celecoxib daily did not increase the CV risk, suggesting that it can be used in the treatment of gastric ulcer.

In the present study, the frequency of bloating was higher in the celecoxib treatment group than in the placebo treatment group. However, the CV events were mild and tolerable, and none of the participants withdrew from this trial.

In conclusion, increases in gastroduodenal ulcer and CV events do not occur in subjects who take 200 mg celecoxib twice daily for two years. Celecoxib can be used in prevention and treatment of gastric cancer.

COMMENTS
Background

Celecoxib, a cyclooxygenase-2 inhibitor, is widely used as an analgesic and anti-inflammatory agent. In addition, it can prevent cancer. However, it is necessary to evaluate the risk of gastroduodenal ulcer and cardiovascular events, particularly in population-based studies.

Research frontiers

No increase in gastroduodenal ulcer and cardiovascular (CV) events were found in the subjects who took 200 mg celecoxib twice daily for two years.

Innovations and breakthroughs

This paper firstly reports an assessment of celecoxib-related gastroduodenal ulcer and cardiovascular events in Chinese population.

Applications

Celecoxib (200 mg twice daily for two years) can prevent and treat gastric cancer in Chinese population.

Peer review

The authors documented the absence of adverse effects of prolonged celecoxib administration at gastroduodenal and cardiovascular level in Chinese patients. The study was well designed and the results are reliable.

Footnotes

Supported by (in part) Grants from National High Technology R&D Program (No. 2002BA711A06), National “211” Project in Peking University 529 and 533, Beijing Municipal Commission of Science and Technology (No. H209-20030130), National Natural Science Foundation of China (No. 30471957), Research Grant Council Earmarked Grant (HKU 7256/01M) of the Hong Kong Special Administration Region, and Research Grant from Peking University School of Oncology, Beijing Cancer Hospital & Institute, China

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