Expression of growth hormone receptor and its mRNA in hepatic cirrhosis

Figure 1 Scatchard analysis of GH-binding in rat liver tissue. Microsomes of liver tissues were incubated with ¹²⁵I-labeled hGH and increased concentrations of unlabeled hGH. The bound/free radioactivity ratio (Y axis) was plotted as a rectilineal func-tion of bound hormone (X axis). GH binding capacities (Bmax = intercept on X axis, R_T = Bmax/protein concentration) and affinities (Kd = negative reciprocal of slope) were calculated from the competition assays by Scatchard analysis.

Figure 2 Correlation between R_T (quantity of GHR) and RCC (degree of cirrhosis) in rat cirrhotic liver samples.

Figure 3 Comparison of GHR mRNA expression in liver tis-sue in normal control group and cirrhotic model group (the result of RT-PCR). Lane M: 100 bp DNA ladder; Lane 1: Nor-mal control group; Lane 2: Cirrhotic model group.