Expression of growth hormone receptor and its mRNA in hepatic cirrhosis

Figure 1 Scatchard analysis of GH-binding in rat liver tissue. Microsomes of liver tissues were incubated with ¹²⁵I-labeled hGH and increased concentrations of unlabeled hGH. The bound/free radioactivity ratio (Y axis) was plotted as a rectilineal func-tion of bound hormone (X axis). GH binding capacities (Bmax = intercept on X axis, R_T = Bmax/protein concentration) and affinities (Kd = negative reciprocal of slope) were calculated from the competition assays by Scatchard analysis.