Codon 249 mutation in exon 7 of p53 gene in plasma DNA: maybe a new early diagnostic marker of hepatocellular carcinoma in Qidong risk area, China

Figure 1 The electrophoresis map of PCR products. Lane 1: DNA Molecular weight markers. Lane 2-9: PCR products of partial samples.

Figure 2 Mutation at coden 249 was identified by restriction digestion. Lane 1: negative control, Lane 2: positive control, Lane 3-9: HCC samples, Lane 10: DNA molecular weight markers.

Figure 3 Sequencing results (partial electropherogram). A: Wild type sequence shows codon 249 AGG; B: Mutation sequence shows codon 249 AGG→AGT transversion; C: Mutation sequence shows codon 249 AGG→AGC transversion; D: A→C transver-sion at the second base of codon 258.