The microcell mediated transfer of human chromosome 8 into highly metastatic rat liver cancer cell line C5F

Figure 1 Double selection of microcell hybrids in HAT and G418 (10 × 25 magnification).

Figure 2 Single-cell isolation cloning in 96-well cell culture plate (10 × 25 magnification).

Figure 3 STS-PCR Amplification of D8S277. 1: chromosome donor cell line A9(neo8); 2: recipient cell line C5F; 3-7: microcell hybrids neo8/C5F-1-5; M: 100bp ladders.

Figure 4 WCP-FISH analysis of neo8/C5F microcell hybrids.

Figure 5 WCP-FISH of neo8/C5F microcell hybrids DAPI counterstain.