Viral Hepatitis
Copyright ©The Author(s) 2003.
World J Gastroenterol. Oct 15, 2003; 9(10): 2211-2215
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2211
Figure 1
Open in New Tab Full Size Figure Download Figure
Figure 1 Structure of plasmid p1301E2.
Figure 2
Open in New Tab Full Size Figure Download Figure
Figure 2 Transgenic plants in greenhouse. A: Flowers of transformants, B: Fruits of transformants.
Figure 3
Open in New Tab Full Size Figure Download Figure
Figure 3 Analysis of Gus gene expression. A: Leaf of untransformed tomato plant, B: Leaf of transformed tomato plant, C: Flesh of untransformed tomato, D: Flesh of trans-formed tomato.
Figure 4
Open in New Tab Full Size Figure Download Figure
Figure 4 Analysis of HEV-E2 gene integration. A: PCR amplifi-cation of genomic DNA of tomato plants, B: Southern dot blot-ting of genomic DNA of tomato plants. A1: DL-2000 marker, A2: Positive control (p1301E2), A3: Total DNA of wild-type con-trol plant, A4-A10: Total DNA of independent transformants. B1: Total DNA of wild-type control plant, B2: Positive control (p1301E2), B3-B8: Total DNA of independent transformants.
Figure 5
Open in New Tab Full Size Figure Download Figure
Figure 5 HEV-E2 expression levels in transgenic tomato plants. 1: Leaves of wild-type control plant, 2-8: Leaves of seven inde-pendent transformants, 9: The mixed flesh tissue of 3 indepen-dent transformant fruits.