Expression of MTLC gene in gastric carcinoma

Figure 1 RT-PCR products were electrophoresed on 1% aga-rose gel containing ethidium bromide. The level of β-actin was used as internal control. M: DL2000 DNA marker; C: gastric cancer tissue; N: adjacent normal gastric tissue.

Figure 2 Cells were determined by manual counting daily. The data was analyzed by Microsoft Excel.

Figure 3 Apoptosis of cells were detected by TUNEL (TdT-mediated dUTP Nick-End Labeling) assay. Green fluorescence of fluores-cein-12-dUTP was detected in apoptotic cells, whereas red fluorescence of PI was observed in all cells. Both signals in a same field were photographed respectively. A: MTLC-expressing cells (× 400); B: control cells (× 400). C: a single apoptotic cell (× 1000).