Copyright
©The Author(s) 2002.
World J Gastroenterol. Apr 15, 2002; 8(2): 258-262
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.258
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.258
Figure 1 Detection of soluble MC3 ScFv in periplasmatic extract by Western blot.
Lanes 1-4: periplasmatic extracts derived from phage clones 12, 19, 23 and 30 respectively, diluted 1:4 with PBS; Lane 5: low molecular weight protein marker.
Figure 2 Detection of soluble MC3 ScFv in TCA-precipitated extracellular fraction by Western blot.
Lane 1: low molecular weight protein marker; Lane 2-5: TCA-precipitated extracellular fractions (to 1/5 of the original volume) derived from the phage clones 12, 19, 23 and 30 respectively.
- Citation: He FT, Nie YZ, Chen BJ, Qiao TD, Fan DM, Li RF, Kang YS, Zhang Y. Expression and identification of recombinant soluble single-chain variable fragment of monoclonal antibody MC3. World J Gastroenterol 2002; 8(2): 258-262
- URL: https://www.wjgnet.com/1007-9327/full/v8/i2/258.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i2.258