Expression and bioactivity identification of soluble MG7 scFv

Figure 1 Restriction analysis of the strong positive recombinant phage clones. M: λ/i I and i II; 1,2: Recombinant clones

Figure 2 Immunodoting of soluble MG₇ scFv. 1: Perplasmic extracts from induced E.coli HB2151; 2: Supernatant from induced E.coli HB2151; 3: Perplasmic extracts from non-induced E.coli HB2151

Figure 3 Western blot of the molecular mass of soluble MG₇ scFv. M: Protein marker; 1: Perplasmic extracts from induced E.coli HB2151; 2: Supernatant from induced E.coli HB2151; 3: Perplasmic extracts from non-induced E.coli HB2151

Figure 4 Nucleotide sequence of MG₇ scFv.