Original Research
Copyright ©The Author(s) 2001.
World J Gastroenterol. Aug 15, 2001; 7(4): 515-521
Published online Aug 15, 2001. doi: 10.3748/wjg.v7.i4.515
Figure 1
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Figure 1 p16 protein expression in normal gastric mucosa. × 400
Figure 2
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Figure 2 p16 protein expression in dysplastic gastric mucosa. × 400
Figure 3
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Figure 3 p16 protein expression in gastric carcinoma. Immunohistochemic stain. Arrow shows the undifferentiated carcinoma positive cell. × 400
Figure 4
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Figure 4 PCR amplification product in exon 2 of p16 gene. Lines 1-4, 5-6, 8-10 (T: gastric cancer); Line 7 (P: tumor adjacent tissue); Line 4 (N: normal gastric mucosa); Line 11 (M: marker) Little PCR product in line 3 and no PCR product in line 5.
Figure 5
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Figure 5 The exon 2 of p16 gene analyzed by SSCP. segment a 135 bp, segment b 249 bp. Lines 2, 3, 5, 6, 7 (T: gastric cancer); Line 4 (P: tumor adjacent tissue); Line 1 (N: normal gastric mucosa). No electrophoresis band on line 3, weak electrophoresis band on line 5, and no abnormal electrophoresis band in all lines.
Figure 6
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Figure 6 The length of PCR amplification of p16 gene exon 2 and the Sam I (CCCGGG) restriction site.