Polymorphism of p16INK4a gene and rare mutation of p15INK4b gene exon2 in primary hepatocarcinoma

Figure 1 PCR-SSCP analysis of p16 gene intron 1 and exon 2. At the SSCP analysis T₂₀ and C₂₀ showed as A pattern; T₂₃ and C₂₃ showed as B pattern; T₂₁ and C₂₁ showed as B' and B pattern, respectively; T₂₂ and C₂₂ showed as B and B' pattern, respectively. T = Human hepatocarcinoma; C = Adjacent non-cancerous liver cirrhosis; N = Normal human leucocyte

Figure 2 PCR product analysis of p15 gene exon 2 in human hepatocarcinoma on agarose gel. M = pBR322/Hea III; N = Normal human leukocyte; T = Human hepatocarcinoma; C = Adjacent non-cancerous liver cirrhosis

Figure 3 PCR-SSCP analysis of p15 gene exon 2. N = Normal human leukocyte; T = Human hepatocarcinoma; C = Adjacent non-cancerous liver cirrhosis

Figure 4 Restriction enzyme analysis of recombinant plasmid which contains aberrant single strand of p15 gene exon 2. M = pBR322/Hea III; 1 = Vector (pUC118) digested with EcoR I and Hind III; 2 = pP15E₂ digested with EcoR I and Hind III

Figure 5 The sequence of p15 gene exon 2 in pP15E₂ recombinant plasmid.