Construction of HBV-specific ribozyme and its recombinant with HDV and their cleavage activity in vitro

Figure 1 Construction of HBV-specific ribozyme gene (RZ), its recombinant with HDV cDNA (DVRZ) and target DNA (BVCF) by PCR. a: Lane 1: PCR marker, Lane 2: ribozyme gene RZ (53 bp). b: Lane 1: λDNA/Hind III + EcoR I marker, Lane 2: recombinant DVRZ (1682 bp), Lane 3: target gene BVCF (831 bp).

Figure 2 In vitro transcription of linearized pTA-RZ, pTA-DVRZ and pTA-BVCF (Lane 1: rDVRZ, 1782 nt. Lane 2: rBVCF, 931 nt. Lane 3: rRZ, 153 nt).

Figure 3 Cleavage of target RNA (rBVCF) catalyzed by rRZ and rDVRZ. a: Cleavage of target RNA (rBVCF) by rRZ under 37 °C, 42 °C and 55 °C with 20 mmol/L Mg²⁺. b: Cleavage of target RNA (rBVCF) by rDVRZ under 37 °C, 42 °C and 55 °C with 20 mmol/L Mg²⁺. c: Cleavage of target RNA (rBVCF) separately by rRZ and rDVRZ at 37 °C under different Mg²⁺ concentrations (10 mmol/L, 15 mmol/L) (Lane 1, 2: cleavage by rRZ separately under 10 and 15 mmol/L Mg²⁺; Lane 3, 4: cleavage by rDVRZ separately under 10 and 15 mmol/L Mg²⁺)