Cloning and expression of HLA-B7 gene

Math 1 Math(A1).

Figure 1 Construction procedure of eukaryotic expression vector.

Figure 2 The electrophoresis results of RT-PCR of HLA-B7 cDNA. 1. λDNA/Hind III marker; 2. The full-length of cDNA (1.5 kb); 3. The upstream region of cDNA (750 bp); 6. The downstream region of cDNA (750 bp)

Figure 3 The results of restriction analysis of pUC-B7. A. 1. λDNA/Hind III marker; 2. pUC19/Hind III; 3. pUC-B7/Hind I + EcoR I; B. 1. pGEM/Hinf I + Rsa I + Sin I marker; 2. cDNA/Hinf I; 3. cDNA (1.5 kb)

Figure 4 The results of PCR detection of pUC-B7; 1. Full length of cDNA (1.5 kb); 2. The upstream region of cDNA (750 bp); 3. The downstream region of cDNA (750 bp); 4. λDNA/Hind III marker

Figure 5 The expression of pCD-B7 in NIH/3T3 cells. A. Negative control; B. Positive cells

Figure 6 The results of pCD-B7 expressed in subcutaneous tissue of mice. A. Negative control; B. Positive cells