Copyright
©The Author(s) 1999.
World J Gastroenterol. Jun 15, 1999; 5(3): 241-244
Published online Jun 15, 1999. doi: 10.3748/wjg.v5.i3.241
Published online Jun 15, 1999. doi: 10.3748/wjg.v5.i3.241
Figure 1 Amplification of human IP-10 and murine crg-2 gene by PCR.
1. crg-2 gene fragment (306 bp); 2. IP-10 gene fragment (314 bp); 3. 100 bp PCR marker (1500, 1200, 1000, 900, 800, 700, 600, 500, 400, 300, 20 and 100 bp fragment, from top to bottom. The 500 and 1000 bp fragme nt serve as reference bands).
Figure 2 Identification of pUC 19/IP-10 and pGE M3Zf(+)/crg-2 recombinant clones by restriction endonucleases digestion.
1. pUC19/IP-10 control; 2. pUC19/IP-10 by Xba-I+Eco R I; 3. pUC 19/IP-10 by Hin d III+Bgl II; 4. 100bp PCR marker; 5. pGEM3Zf (+)/crg-2 control; 6. pGEM3Zf(+)/crg-2 by Hin d III; 7. pGEM3Zf (+)/crg-2 by Bam H I.
Figure 3 The nucleotide sequence of IP-10/crg-2 gene encoding region.
Human IP-10 sequence (above); murine-crg-2 sequencing (below).
- Citation: Liu ZG, Yang JH, An HZ, Wang HY, He FT, Han ZY, Han Y, Wu HP, Xiao B, Fan DM. Cloning and identification of an angiostatic molecule IP-10/crg-2. World J Gastroenterol 1999; 5(3): 241-244
- URL: https://www.wjgnet.com/1007-9327/full/v5/i3/241.htm
- DOI: https://dx.doi.org/10.3748/wjg.v5.i3.241