Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration

Figure 1 Schematic and histological figures of liver lobule. A: Schematic illustration of mutual compatibility of “classical” and “portal” lobules with hepatic acini. I, II, III – the zones of the acinus. The hatched periportal area “a” – localized in the 1^st zone of the acinus. The hatched pericentral area “b” – localized in the 3^rd zone of the acinus. Orange arrow – central vein; black arrow – portal triad. B: Pericentral hepatocytes (CK8) ObX40; OcX20; C: Periportal hepatocytes (CK8). ObX40; OcX20.

Figure 2 Hepatocytes’ ploidy in normal (control), regenerated (SG₁) and re-regeneration (SG₂).

Figure 3 Histology and scanning electron microscopy of corrosion casts of the livers from control group. A and B: Liver lobules histology (H&E); C: Liver lobule (histology after Indian ink – gelatin injection); D: Adjacent lobules with intercommunicated sinusoidal meshwork (histology after Indian ink – gelatin injection); E: Connective-tissue sheath (arrow) around the tributary of hepatic vein (Masson’s Trichrome); F: Scanning electron microscopy (SEM) of corrosion casts of small branches of portal vein and sinusoids; periportal plexus (arrow); G-L: SEM of corrosion casts of sinusoids and related vessels; J and K: SEM of corrosion casts of superficial (sub-capsular) vessels of the liver.

Figure 4 Histology and scanning electron microscopy of corrosion casts of regenerated liver (SG₁). A and B: Liver tissue histology (Masson’s Trichrome); C: Liver lobules (histology after Indian ink – gelatin injection); D: “mega-lobule” formed by adjacent lobules with intercommunicated sinusoidal meshwork (bordered by yellow line) (histology after Indian ink – gelatin injection); E-J: Scanning electron microscopy (SEM) of corrosion casts of liver vessels; E and F: “mega-lobules”; G-I: SEM of corrosion casts of sinusoids; J: SEM of vascular corrosion casts of liver lobe; “mega-lobules” (bordered by red line).

Figure 5 Histology and scanning electron microscopy of corrosion casts of livers from re-regenerated liver (SG₂). A: Liver tissue histology (H&E); B and D: Connective-tissue fibers around the portal triad and perilobular branches (Masson’s Trichrome); C: Liver lobules (histology after Indian ink – gelatin injection); E-I: Scanning electron microscopy (SEM) of liver vascular corrosion casts; E and F: “mega-lobules”; G-I: SEM of corrosion casts of sinusoids

Figure 6 Ductular reaction. A and B: Portal/periportal ductular reaction in SG₁ (CK8); C: Intralobular ductular reaction in SG₁ (H&E); D-F: Intralobular ductular reaction in SG₂ (CK8).