Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Dec 21, 2019; 25(47): 6823-6834
Published online Dec 21, 2019. doi: 10.3748/wjg.v25.i47.6823
Figure 1
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Figure 1 Differentially expressed miRNAs between gastric adenocarcinoma sample and normal sample. A: Volcano plot for differentially expressed miRNA (DEM) expression. Dark dots represent upregulated miRNAs, whereas lighter dots represent downregulated miRNAs; B: Hierarchical gene clustering analysis of DEMs represented by a heat map. Dark represents upregulated miRNAs, whereas lighter represents downregulated miRNAs.
Figure 2
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Figure 2 Association of differentially expressed miRNAs with overall survival of gastric adenocarcinoma. Dark lines represent high expression, and lighter lines represent low expression.
Figure 3
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Figure 3 The identification of differentially expressed miRNA levels in gastric adenocarcinoma patients and healthy subjects. A: The levels of miR-96-5p, miR-222-5p, miR-652-5p, miR-125-5p, miR-145-3p, and miR-379-3p in tumor samples and adjacent normal samples by qRT-PCR; B: The miR-96-5p level in the blood of gastric adenocarcinoma patients and healthy subjects by qRT-PCR (bP < 0.01). Values are mean ± SD.
Figure 4
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Figure 4 Target gene prediction and identification of miR-96-5p. A: The intersection of the predicting target genes of miR-96-5p among the TargetScan, miRTarBase and miRDB databases using a Venn diagram; B: Prediction of the binding site of miR-96-5p to zinc finger DHHC-type palmitoyltransferase 5 (ZDHHC5) by bioinformatics; C: Target regulation of miR-96-5p to ZDHHC5 observed by the luciferase reporter system. ZDHHC5: Zinc finger DHHC-type palmitoyltransferase 5.
Figure 5
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Figure 5 miR-96-5p inhibition might induce cell apoptosis in MGC-803 cells. A: The number of apoptotic cells after transfection with inhibitor NC, miR-96-5p inhibitor, si-zinc finger DHHC-type palmitoyltransferase 5 (ZDHHC5), or miR-96-5p inhibitor + si-ZDHHC5 in MGC-803 cells by flow cytometry analysis; B: The expression of ZDHHC5, Bcl-2, and COX2 after cells were treated with inhibitor NC, miR-96-5p inhibitor, si-ZDHHC5, or miR-96-5p inhibitor + si-ZDHHC5 in MGC-803 cells by western blotting (aP < 0.01). Values are mean ± SD. ZDHHC5: Zinc finger DHHC-type palmitoyltransferase 5.