Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Apr 28, 2019; 25(16): 1964-1974
Published online Apr 28, 2019. doi: 10.3748/wjg.v25.i16.1964
Figure 1
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Figure 1 The structure and location of circ-PRKCI. Circ-PRKCI is a closed circular RNA located on chromosome 3.
Figure 2
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Figure 2 The level of circ-PRKCI. A: The expression level of circ-PRKCI in cancer and para-carcinoma tissues. Its expression level was higher in cancer tissue; B: The expression level of circ-PRKCI in cancer tissue and normal human blood. There was no significant difference in the level of expression; C: The expression of circ-PRKCI in different cell lines. The expression levels were different in different cell lines; D: When over-confluence was maintained, the content of circ-PRKCI decreased significantly. bP < 0.01.
Figure 3
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Figure 3 Effect of circ-PRKCI on the growth of HepG2 cells. A: Two specific siRNAs targeting circ-PRKCI; B: Rnase R enzyme digestion of linear PRKCI, and circ-PRKCI silencing by specific si-RNA1 and si-RNA2; C: After circ-PRKCI silencing, the apoptosis of tumor cells was increased (bP < 0.01); D: The expression plasmid and control plasmid of circ-PRKCI were constructed. E: Overexpression of circ-PRKCI increased the invasiveness of cancer cells, while circ-PRKCI silencing significantly decreased their invasiveness; F: Cell proliferation after silencing or overexpression of circ-PRKCI. CMV: Cytomegalovirus; GFP: Green fluorescent protein.
Figure 4
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Figure 4 Circ-PRKCI regulates cell function through the AKT3 signaling pathway. A: Subcellular localization of circ-PRKCI. Fluorescence in situ hybridization revealed that it is localized in the cytoplasm; B: Overexpression of circ-PRKCI reduces the expression of AKT3 protein. Con: Control; AKT3: AKT serine/threonine kinase 3.
Figure 5
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Figure 5 E2F transcription factor 7 affects survival rate of patients with liver cancer. The higher the expression level of E2F transcription factor 7, the lower the survival rate of patients. When the survival rate was 50%, the survival time of the high expression group was about 82 mo, and that of the low expression group was about 30 mo. “Survival” means only cancer related death. E2F7: E2F transcription factor 7; HR: Hazard ratio.