Regulating effect of TongXie-YaoFang on colonic epithelial secretion via Cl- and HCO3- channel

Figure 1 Comparison of basic electrophysiological properties of colonic mucosa in rats and 5-HT-induced the changes of short-circuit current among three groups. A-C: The basic Isc, basic voltage and basic resistance did not differ significantly between three groups, respectively (P > 0.05, respectively); D: TXYF-formula significantly increased 5-HT-induced ∆Isc in neonatal maternal separation plus restraint stress (NMS + RS) rats. ^bP < 0.01 vs NMS + RS group. Isc: The short-circuit current; ∆Isc: The changes of Isc; TXYF: TongXie-YaoFang; NMS: Neonatal maternal separation; RS: Restraint stress; NH: No handlings as control.

Figure 2 Effects of 1,1-dimethyl piperidinium chloride, Glibenclamide, cystic fibrosis transmembrane conductance regulator(inh)-172, 4,4’-diisothiocyanato-stilbene-2,2’-disulfonic acid, Amiloride and Bumetanide on 5-HT-induced the changes of short-circuit current in rats. A: After the intervention with DPC, Glibenclamide or CFTR(inh)-172, respectively, ∆Isc induced by 5-HT were similar in three groups (P > 0.05, respectively); B: 5-HT-induced ∆Isc was higher in TXYF-formula group than in NMS + RS group after the intervention with DIDS or Amiloride. ^aP < 0.05 vs NMS + RS group; ^bP < 0.01 vs NMS + RS group. DPC: 1,1-dimethyl piperidinium chloride; CFTR: Cystic fibrosis transmembrane conductance regulator; ∆Isc: the changes short-circuit current; DIDS: 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid; TXYF: TongXie-YaoFang; NMS: Neonatal maternal separation; RS: Restraint stress; NH: No handlings as control.

Figure 3 Effects of BaCl₂, 4-acetamido-4’-isothio-cyanato-stilbene-2,2’-disulfonic and 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid on 5-HT-induced the changes of short-circuit current in rats. 5-HT-induced ∆Isc were similar in three groups after the intervention with BaCl₂, SITS or DIDS, respectively (P > 0.05, respectively). ∆Isc: the changes short-circuit current; SITS: 4-acetamido-4′-isothio-cyanato-stilbene-2,2′-disulfonic; DIDS: 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid; TXYF: TongXie-YaoFang; NMS: Neonatal maternal separation; RS: Restraint stress; NH: No handlings as control.

Figure 4 Effects of Na⁺, Cl^– and HCO₃^– on 5-HT-induced the changes of short-circuit current in rats. A: 5-HT-induced ∆Isc was higher in TXYF-formula group than in NMS + RS group when Na⁺ in apical side of colonic mucosa was taken out; B: When Na⁺ in basolateral side was taken out, ∆Isc was similar in three groups; C: 5-HT-induced ∆Isc was similar in three groups when Cl^– and HCO₃^– was respectively or entirely removed from basolateral side of colonic mucosa. ^aP < 0.05 vs NMS + RS group; ^bP < 0.01 vs NMS + RS group. ∆Isc: the changes short-circuit current; TXYF: TongXie-YaoFang; NMS: Neonatal maternal separation; RS: Restraint stress; NH: No handlings as control.